Search Results

You are looking at 1 - 4 of 4 items for

  • Author or Editor: Scott B. Citino x
  • Refine by Access: All Content x
Clear All Modify Search

Abstract

Objective—To analyze the 7a7b genes of the feline coronavirus (FCoV) of cheetahs, which are believed to play a role in virulence of this virus.

Sample Population—Biologic samples collected during a 4-year period from 5 cheetahs at the same institution and at 1 time point from 4 cheetahs at different institutions.

Procedures—Samples were first screened for FCoV via a reverse transcription-PCR procedure involving primers that encompassed the 3′-untranslated region. Samples that yielded positive assay results were analyzed by use of primers that targeted the 7a7b open reading frames. The nucleotide sequences of the 7a7b amplification products were determined and analyzed.

Results—In most isolates, substantial deletional mutations in the 7a gene were detected that would result in aberrant or no expression of the 7a product because of altered reading frames. Although the 7b gene was also found to contain mutations, these were primarily point mutations resulting in minor amino acid changes. The coronavirus associated with 1 cheetah with feline infectious peritonitis had intact 7a and 7b genes.

Conclusions and Clinical Relevance—The data suggest that mutations arise readily in the 7a region and may remain stable in FCoV of cheetahs. In contrast, an intact 7b gene may be necessary for in vivo virus infection and replication. Persistent infection with FCoV in a cheetah population results in continued virus circulation and may lead to a quasispecies of virus variants.

Restricted access
in American Journal of Veterinary Research

Abstract

Objective—To determine ECG and echocardiographic measurements in healthy anesthetized Grevy's zebras (Equus grevyi).

Animals—20 healthy zebras.

Procedures—Auscultation, base-apex ECG, and echocardiography were performed on anesthetized zebras.

Results—Low-grade systolic murmurs were detected in the left basilar region in 4 of 20 zebras. Evaluation of ECGs from 19 zebras revealed sinus rhythm with a predominantly negative QRS complex and a mean ± SD heart rate of 67 ± 10 beats/min. Echocardiograms of sufficient image quality were obtained for 16 zebras. Interventricular septal thickness in diastole, left ventricular chamber in diastole and systole, left atrial diameter, and left ventricular mass were significantly and moderately correlated with estimated body weight (r values ranged from 0.650 to 0.884). Detectable swirling of blood in the right and sometimes the left ventricles was detected in 9 of 16 zebras, whereas physiologic regurgitation of blood was detected for the aortic valve in 3 zebras, pulmonary valve in 2 zebras, mitral valve in 2 zebras, and tricuspid valve in 1 zebra.

Conclusions and Clinical Relevance—Results of this study provide reference information for use in the cardiac evaluation of anesthetized Grevy's zebras.

Restricted access
in American Journal of Veterinary Research

Abstract

OBJECTIVE To determine the incidence of and risk factors for clinical feline herpesvirus (FHV) infection in zoo-housed cheetahs and determine whether dam infection was associated with offspring infection.

DESIGN Retrospective cohort study.

ANIMALS 144 cheetah cubs born in 6 zoos from 1988 through 2007.

PROCEDURES Data were extracted from the health records of cheetahs and their dams to identify incident cases of clinical FHV infection and estimate incidence from birth to 18 months of age. Univariate and multivariable Cox proportional hazards models, controlling for correlations among cheetahs with the same dam, were used to identify risk factors for incident FHV infection.

RESULTS Cumulative incidence of FHV infection in cheetah cubs was 35% (50/144). No significant association between dam and offspring infection was identified in any model. Factors identified as significant through multivariable analysis varied by age group. For cheetahs up to 3 months of age, the most important predictor of FHV infection was having a dam that had received a preparturition FHV vaccine regimen that included a modified-live virus vaccine versus a dam that had received no preparturition vaccine. Other risk factors included being from a small litter, being born to a primiparous dam, and male sex.

CONCLUSIONS AND CLINICAL RELEVANCE This study provided the first population-level characterization of the incidence of and risk factors for FHV infection in cheetahs, and findings confirmed the importance of this disease. Recognition that clinical FHV infection in the dam was not a significant predictor of disease in cubs and identification of other significant factors have implications for disease management.

Restricted access
in Journal of the American Veterinary Medical Association
Tending Animals in the Global Village—A Guide to International Veterinary Medicine . . . . Import Risk Analysis: Animals and Animal Products . . . . Exotic Pests & Disease: Biology and Economics for Biosecurity . . . . Borna Disease Virus and Its Role in Neurobehavioral Disease . . . . Foot and Mouth Disease: Facing the New Dilemmas . . . . Trends in Emerging Viral Infections of Swine . . . . Clinical Examination of Farm Animals . . . . Manual of Sheep Diseases (2nd edition) . . . . Mycotoxins: Risks in Plant, Animal, and Human Systems . . . . A Guide to Plant Poisoning of Animals in North America . . . . Diseases of Poultry (11th edition) . . . . Modern Concepts of Immunology in Veterinary Medicine—Poultry Immunology (Advances in Medical and Veterinary Immunology) . . . . Pathology of Pet and Aviary Birds . . . . Birds of Prey: Health and Disease (3rd edition) . . . . Hand-Rearing Wild and Domestic Mammals . . . . Handbook of Wildlife Chemical Immobilization (International Edition) . . . . Veterinary Anesthesia and Pain Management Secrets . . . . The Veterinary ICU Book . . . . Anatomy of the Dog: An Illustrated Text (4th Edition) . . . . The 5-Minute Veterinary Consult Clinical Companion: Small Animal Dermatology . . . . Abdominal Radiology for the Small Animal Practitioner (Made Easy Series) . . . . Two Dimensional and M-Mode Echocardiography for the Small Animal Practitioner (Made Easy Series) . . . . Small Animal Ophthalmology Secrets . . . . Ocular Tumors in Animals and Humans . . . . Feline Oncology: A Comprehensive Guide to Compassionate Care . . . . Veterinary Medicine and Practice 25 Years in the Future and the Economic Steps to Get There
Restricted access
in Journal of the American Veterinary Medical Association