Objective—To evaluate quality of duodenal tissue
specimens obtained endoscopically from dogs and
cats and submitted to 1 of 2 diagnostic laboratories
Sample Population—Slides from 50 consecutive
canine and 50 consecutive feline endoscopically
obtained duodenal tissue specimens submitted to
laboratory 1 and 49 consecutive canine and 46 consecutive
feline specimens submitted to laboratory 2.
Procedure—Slides were examined independently by
3 investigators, and each tissue piece on each slide
was classified as clearly inadequate, questionable, or
clearly adequate on the basis of 4 criteria. An overall
score was then assigned to the slide.
Results—Slides from laboratory 1 were more likely to
be scored as clearly adequate and less likely to be
scored as clearly inadequate than slides from laboratory
2. Clearly adequate slides from laboratory 1 had
a higher number of clearly adequate pieces of tissue
than did clearly adequate slides from laboratory 2.
Slides scored as clearly adequate had a higher number
of individual tissue pieces than did slides scored
as clearly inadequate.
Conclusions and Clinical Relevance—Results suggest
that the quality of endoscopically obtained duodenal
tissue specimens submitted to laboratories can
vary, possibly because of differences in experience of
individuals collecting biopsy specimens. Results suggest
that at least 8 individual tissue pieces should be
submitted when performing endoscopic biopsy of the
duodenum in dogs and cats. (J Am Vet Med Assoc