Objective—To identify microsatellite markers linked
to progressive retinal atrophy (PRA) in American
Sample Population—Blood samples or buccal
epithelial cells from 66 American Eskimo Dogs,
including 53 PRA-unaffected and 13 PRA-affected
Procedure—The genotypes of unaffected and affected
dogs were determined by use of microsatellite
markers spanning canine chromosome 9 (CFA09).
Homozygosity mapping was used to detect linkage
between markers and the gene locus for PRA.
Results—Significant allelic association between
marker alleles and the gene locus for PRA was
detected for GALK1 and TK1, indicating linkage
between these markers and the causative gene locus
Conclusions and Clinical Relevance—These data
indicate that PRA in American Eskimo Dogs is located
on CFA09 and allow for the development of a
microsatellite-based test to identify carrier (unaffected)
and affected dogs before clinical signs appear.
(Am J Vet Res 2005;66:1900–1902)