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  • Author or Editor: Ronald D. Welsh x
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in Journal of the American Veterinary Medical Association

Abstract

Objective—To evaluate a method using Proteus mirabilis and Pseudomonas aeruginosa to experimentally induce dual infection of the urinary bladder in dogs.

Animals—6 healthy mixed-breed dogs.

Procedure—Dogs were anesthetized, and cystitis was induced by infusing a solution of salicylic acid in ethanol into the bladder, followed by an inoculum containing field isolates of P mirabilis and P aeruginosa. Dogs were examined daily for 21 days after induction of cystitis. On day 21, dogs were euthanatized, and urinary bladder, renal pelvis, and prostate specimens were submitted for bacterial culture.

Results—After induction of cystitis, all dogs had evidence of thickening of the bladder wall, dysuria, tenesmus, and hematuria. Urinalysis revealed proteinuria, hematuria, and pyuria. All urine samples obtained on day 21 yielded growth of P mirabilis, but P aeruginosa was not cultured from any of these samples. Proteus mirabilis was isolated from bladder, renal pelvis, or prostate specimens from 4 dogs; P aeruginosa was not isolated from any of the tissue specimens.

Conclusion—Results suggest that the method used in the present study fails to induce dual infection of the urinary bladder with P mirabilis and P aeruginosa. The inability to establish a persistent dual infection with this method may have been a result of insufficient pathogenicity of the Pseudomonas isolate or an inadequacy of the experimental design. (Am J Vet Res 2000;61:1484–1486)

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in American Journal of Veterinary Research

Abstract

Objective—To assess the effects of porcine small intestinal submucosa (SIS) implants on the healing of meniscal lesions in dogs.

Animals—16 adult Greyhounds of both sexes.

Procedure—Unilateral osteotomy was performed at time 0 to disrupt the medial collateral ligament attachment, and two (1 cranial and 1 caudal) 4-mm circular defects were created in the avascular portion of the medial meniscus. One defect was filled with an SIS graft, and the other defect remained empty (control). Three months later, the identical procedure was performed on the contralateral limb. Three months after the second surgery, dogs were euthanatized, and meniscal tissue specimens from both stifle joints were collected for gross, histologic, biomechanical, and biochemical evaluations.

Results—Regenerative tissue was evident in 4 (2 SIS-implanted and 2 control) of 16 defects examined histologically. In 3 defects, this thin bridge of tissue was composed of immature haphazardly arranged fibrous connective tissue with a relatively uniform distribution of fibroblasts. Aggregate modulus, Poisson ratio, permeability, and shear modulus were not significantly different between control and SIS-implanted defects either 3 or 6 months after surgery. Hydroxyproline content also did not differ between SIS-implanted and control defects at 3 or 6 months.

Conclusions and Clinical Relevance—Implantation of porcine SIS into experimentally induced meniscal lesions in dogs did not promote tissue regeneration. (Am J Vet Res 2002;63:427–431)

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in American Journal of Veterinary Research