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  • Author or Editor: Richard Weilenmann x
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Objective—To evaluate a modified posterior rhinomanometric method for clinical application in dogs.

Animals—15 healthy Beagles and 8 Bulldogs (4 healthy and 4 with respiratory problems).

Procedures—Rhinomanometry was performed 3 times within a 15-minute period in anesthetized dogs.Transnasal pressure (PNA) and nasal resistance (RNA) were determined by use of artificial airflow (adjusted for body weight) for inspiration (PNAin and RNAin, respectively) and expiration (PNAout and RNAout). Procedures were repeated for the Beagles 7 days later.

Results—For the Beagles, mean ± SD of PNAin for both days (0.162 ± 0.042 kPa) was significantly lower than PNAout (0.183 ± 0.053 kPa). Similarly, RNAin (1.47 ± 0.41 kPa/[L/s]) was significantly lower than RNAout (1.64 ± 0.46 kPa/[L/s]). Pairwise comparison of values for PNA and RNA for the 2 days revealed no significant difference. Repeatability of the method (estimated as within-day variation) for RNA was ± 0.19 kPa/(L/s), whereas variation between the days was ± 0.36 kPa/(L/s) for RNAin and ± 0.44 kPa/(L/s) for RNAout. The 4 clinically normal Bulldogs had RNA values ranging from 1.69 to 3.48 kPa/(L/s), whereas in the 4 Bulldogs with respiratory problems, RNA ranged from 9.83 to 20.27 kPa/(L/s).

Conclusions and Clinical Relevance—RNA is inversely dependent on body size and nonlinearly associated with airflow. We propose that RNA in dogs should be determined for airflows standardized on the basis of body size. The PNA and RNA in Beagles can be measured with sufficient repeatability for clinical use and nasal obstructions are detectable.

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in American Journal of Veterinary Research



To establish an ELISA for detection of serum total IgE concentration in dogs and to analyze IgE values in a dog colony.


147 healthy Beagles (31 males and 116 females).


2 canine IgE-specific polyclonal antibodies elicited by 2 recombinant fragments of the epsilon chain in hens were used to develop a capture ELISA specific for serum total IgE concentration. The IgE values were calculated by comparing serum dose-response curves (1:50 to 1:6,400) with a reference serum pool assigned 100 relative ELISA units (REU).


Mean IgE concentration in female Beagles was 51.2 REU (range, 0 to 337.8 REU; median, 31.4 REU), whereas mean IgE concentration in male dogs was only 7.5 REU (range, 0 to 32.6 REU; mean, 3.6 REU). Distribution of IgE values was skewed; approximately 80% of dogs had IgE values < 50 REU. Analysis of natural logarithmically transformed IgE values indicated that sex and age significantly (P < 0.05) influenced IgE values; mean serum IgE values increased until the age of 4 years. Heritability estimates of IgE concentration indicated a trend toward a genetic influence.


A reliable capture ELISA specific for canine IgE was developed. Serum total IgE values vary with age and sex in the sample population.

Clinical Relevance

Serum total IgE concentration can now be evaluated in various dog breeds and, subsequently, in dogs with IgE-mediated diseases provided that these significant influences are accounted for. Serum total IgE values may then prove to be of diagnostic value, similar to their use in human beings. (Am J Vet Res 1999;60:93–97)

Free access
in American Journal of Veterinary Research