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The Jockey Club of North America requires that all breeding of Thoroughbred racehorses be limited to live matings. Given this restriction, the traditional number of mares bred by a Thoroughbred stallion during a single 5-month breeding season (referred to as a stallion's book) that will enable a stallion to achieve a target pregnancy rate of 75% with a live foaling rate of 60% has been 40 mares. 1 , a It is unclear how this traditional value for a Thoroughbred stallion's book was established, but it is likely that it was simply a historical industry maximum based on practical

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in Journal of the American Veterinary Medical Association


Case Description—2 Standardbred racehorses that had been winning races while competing as mares underwent postrace drug testing and had serum testosterone concentrations above the acceptable limit for female racehorses.

Clinical Findings—Initial physical examinations by the referring veterinarian revealed ambiguous external genitalia and suspected intra-abdominally located testes leading to a preliminary diagnosis of male pseudohermaphroditism. Horses were referred for further evaluation of sex. Physical examination of the external genitalia confirmed the findings of the referring veterinarian. Transrectal palpation and ultrasonography revealed gonads with an ultrasonographic appearance of testes. On cytogenetic analysis, both horses were determined to have a 64,XY karyotype and 8 intact Y chromosome markers and 5 SRY gene markers, which were indicative of a genetic male and confirmed an intersex condition. Additionally, both horses had some male-type behavior and endocrinologic findings consistent with those of sexually intact males.

Treatment and Outcome—Taken together, these findings confirmed that both horses were male pseudohermaphrodites. Both horses returned to racing competition as males.

Clinical Relevance—As of October 1, 2008, the Pennsylvania Horse and Harness Racing Commissions implemented a postrace drug testing policy that included analysis of blood samples for anabolic and androgenic steroids and set maximum allowable concentrations of testosterone for racing geldings and females. Within 8 months of initiation of this drug testing policy, the 2 horses of this report were identified as having an intersex condition. This raises the possibility that intersex conditions may be more common in racing Standardbreds than was previously suspected.

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in Journal of the American Veterinary Medical Association


Objective—To determine whether a homologue of A-kinase anchor protein 4 (AKAP4) is present and functional as an AKAP in equine spermatozoa and examine the effect of semen cooling and cryopreservation on binding of equine AKAP4 to the regulatory (RII) subunit of protein kinase-A (PK-A).

Sample Population—Ejaculated semen collected from 2 fertile stallions, 3 bulls, and 3 humans.

Procedure—Identification of an equine homologue of AKAP4 was investigated via DNA sequencing. Protein was extracted from the spermatozoa of each species for immunoblot analysis to identify AKAP4 and its precursor protein, pro-AKAP4; immunofluorescence microscopy was used to localize those proteins in spermatozoa. Ligand overlay assays were used to determine whether the identified proteins bound to the RII subunit of PK-A and whether cooling or cryopreservation of spermatozoa affected that binding.

Results—The partial genomic sequence of AKAP4 was identified in equine spermatozoa, and immunoblot analysis confirmed that AKAP4 and pro-AKAP4 are present in equine spermatozoa. Via immunofluorescence microscopy, these proteins were localized to the spermatozoal principal piece. Results of ligand overlay assays indicated that equine AKAP4 and pro-AKAP4 bind to the RII subunit of PKA and are AKAPs; AKAP4-RII binding was not affected by cooling or cryopreservation of spermatozoa.

Conclusions and Clinical Relevance—Results suggest that equine AKAP4 anchors PK-A to the spermatozoal flagellum (where the kinase is likely to be required for the regulation of spermatozoal motility), but decreases in spermatozoal motility in cooled or cryopreserved semen are not associated with decreased binding of AKAP4 and PK-A. (Am J Vet Res 2005;66:1056–1064)

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in American Journal of Veterinary Research

A 4-year-old Standardbred stallion was retired from racing because of an injury to a tendon in the right forelimb. The owners intended to sell the stallion for use as a stud. A series of prepurchase semen evaluations conducted by personnel at the breeding farm consistently revealed low spermatozoa motility.

The breeding manager also observed that the horse would intermittently turn its head abruptly and gaze toward its groin. The horse would sometimes bite at its flank, kick out with both hind limbs, and pace in a circle. Since early race training when the stallion was 2 years old, it

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in Journal of the American Veterinary Medical Association