Objective—To determine whether bovine immunodeficiency
virus (BIV) infection could be detected in
spermatozoa, blood leukocytes, or semen leukocytes
from stud bulls in artificial insemination centers.
Animals—30 bulls at 3 artificial insemination centers.
Procedure—Polymerase chain reaction testing that
used 3 sets of primer pairs targeting pol and env
regions of the BIV proviral genome was performed on
DNA extracted from semen leukocytes, spermatozoa,
and blood leukocytes from each bull. Southern blot
analysis was performed to increase sensitivity of
detection. Western blot analysis of plasma samples
was used to detect antibodies against BIV.
Results—BIV provirus was not detected in DNA samples
obtained from semen leukocytes, spermatozoa,
or blood leukocytes, and antibodies against BIV were
Conclusions and Clinical Relevance—Contrary to
our report of high point prevalence of BIV contamination
of semen from a single artificial insemination
center, bulls of the study reported here did not appear
to be infected. Maximum risk of BIV infection in similar
bulls was estimated at 10% with a confidence
level of 95%. (Am J Vet Res 2000;61:816–819)