Objective—To determine whether severity of leukocytosis
correlates with severity of postmortem
lesions in dogs with immune-mediated hemolytic
Animals—34 dogs with IMHA that had CBC performed
within 48 hours prior to death and complete
Procedure—Dogs were independently assigned to 4
leukocytosis groups (within reference range; mild
leukocytosis, moderate leukocytosis, marked leukocytosis)
and 3 lesion severity groups (mild lesions,
moderate lesions, severe lesions).
Results—Moderate to marked leukocytosis correlated
with moderate to severe postmortem lesions.
Ischemic necrosis within liver, kidney, heart, lung, and
spleen attributable to thromboembolic disease or anemic
hypoxia were the most common important
lesions found at necropsy. None of the dogs with mild
lesions had moderate or marked leukocytosis. Four of
14 severely affected dogs had WBC counts within reference
range, but all 4 had neutrophilic left shifts.
Three of these 4 dogs had toxic change in neutrophils.
Conclusion and Clinical Relevance—Moderate to
marked leukocytosis, neutrophilic left shift, and toxic
change in neutrophils in dogs with IMHA should alert
clinicians to the potential for moderate to severe tissue
injury, which could complicate treatment and
worsen prognosis. Lesions appear to be secondary to
anemic hypoxia, thromboembolic disease, or both;
therefore, treatment objectives should focus on
improving blood oxygen-carrying capacity and monitoring
for thromboembolic disease. (J Am Vet Med
Objective—To determine sensitivity and specificity of
physical examination, fine-needle aspiration, and needle
core biopsy of the regional lymph nodes for evidence
of metastasis in dogs and cats with solid
Animals—37 dogs and 7 cats.
Procedure—Regional lymph nodes were evaluated
by means of physical examination (palpation), fineneedle
aspiration, and needle core biopsy. Results
were compared with results of histologic examination
of the entire lymph node, the current standard.
Results—Tumors included 18 sarcomas, 16 carcinomas,
7 mast cell tumors, and 3 other tumors.
Carcinomas were more likely to have metastasized to
the regional lymph node (7/16 animals) than were sarcomas
(2/18). Sensitivity and specificity of physical
examination were 60 and 72%, respectively.
Sensitivity and specificity of cytologic examination of
fine-needle aspirates were 100 and 96%, respectively.
Sensitivity and specificity of histologic examination
of needle core biopsy specimens were 64 and 96%,
Conclusions and Clinical Relevance—Results suggested
that fine-needle aspiration may be a sensitive
and specific method of evaluating the regional lymph
nodes in dogs and cats with solid tumors, because
results correlated well with results of histologic examination
of the entire lymph node. Physical examination
alone was not a reliable method and should not be
used to decide whether to aspirate or biopsy the
regional lymph nodes. (J Am Vet Med Assoc