Objective—To evaluate the effect of platelet-rich plasma on wounds on the distal aspect of the forelimb in horses.
Animals—6 mixed-breed 10- to 15-year-old mares.
Procedures—3 wounds were created on metacarpal regions in each of 6 horses (n = 36 wounds total). Eighteen wounds were treated with platelet-rich plasma and bandaged, whereas 18 control wounds were similarly bandaged with no prior topical treatment. Decrease in wound surface area and the required number of excisions of exuberant granulation tissue were recorded until complete healing. Tissue specimens were taken from wounds at 1 week for histologic examination and measurement of transforming growth factor-β1 concentrations and at closure for histologic examination, biomechanical evaluation, and measurement of collagen type I and type III mRNA.
Results—Platelet-rich plasma favored excessive development of granulation tissue and significantly slowed wound healing at 1, 2, and 3 weeks after surgery. Transforming growth factor-β1 had a 1.6-fold higher concentration in treated wounds, compared with untreated wounds. Histologic, biomechanical, and gene expression data did not differ significantly between treated and control wounds.
Conclusions and Clinical Relevance—Topical application of autologous platelet-rich plasma did not accelerate or improve the quality of repair of small granulating wounds on limbs of horses. This treatment may better suit wounds with massive tissue loss or, alternatively, chronic wounds that would benefit from a fresh source of mediators to accelerate the healing process.
Objective—To compare accuracy of 2 methods for
injection of the podotrochlear bursa in horses.
Animals—17 French Standardbreds.
Procedure—In each horse, contrast medium was
injected into the podotrochlear bursa of 1 foot by
use of a distal palmar approach with the needle
inserted parallel to the sole (DPPS) and into the
podotrochlear bursa of the other foot by use of a distal
palmar approach to the navicular position (DPNP).
Podotrochlear bursa injection was evaluated by
means of radiographic examination in all horses and
postmortem examination in 6.
Results—Contrast medium was successfully injected
into the podotrochlear bursa in 6 of 16 feet in which
the DPPS method was used and 14 of 17 feet in
which the DPNP method was used; these results
were significantly different. Failure was attributed to
contrast medium invading the distal interphalangeal
joint, contrast medium pooling palmar to the deep
digital flexor tendon, contrast medium located in the
podotrochlear bursa and the distal interphalangeal
joint, contrast medium located in the podotrochlear
bursa and the area palmar to the deep digital flexor
tendon, and an inability to inject contrast medium
despite adequate needle placement.
Conclusions and Clinical Relevance—Results suggest
that the DPNP technique can be used successfully
for injection into the podotrochlear bursa in horses.
However, radiographic assessment should be
used to confirm that material was injected into the
bursa. (J Am Vet Med Assoc 2005;226:1524–1528)
Objective—To evaluate the usefulness of multisite
quantitative ultrasonography for noninvasive assessment
of bone in horses.
Sample Population—12 healthy horses and both
forelimbs from 8 clinically normal horses.
Procedure—For in vivo measurements, various
regions of interest (ROI) were examined on the third
metacarpal bone, radius, and tibia. Precision error for
speed of sound (SOS) measurements was obtained
by measuring each ROI of 4 horses 10 times with
probe repositioning. Additionally, 3 operators measured
each aspect of the third metacarpal bone of 6
horses 5 times each. For ex vivo measurements, third
metacarpal bones were examined at 9 ROI, and SOS
measurements were performed before and after soft
tissue removal. One ROI of a single forelimb was subjected
to 96 ex vivo measurements with 3 different
Results—The lateral aspect of the third metacarpal
bone had significantly higher SOS values than the dorsal
and medial aspect of the third metacarpal bone.
No difference was obtained between SOS values of
the lateral and medial aspect of the radius. The tibia
had significantly higher SOS values than the lateral
aspect of the radius and the dorsal and medial aspect
of the third metacarpal bone. Intraoperator coefficients
of variation ranged from 0.62 to 3.15%, and
interoperator coefficients of variation ranged from
0.78 to 2.70%. Values of SOS were highest when silicone
oil was used as the contact medium.
Conclusions and Clinical Relevance—Speed of
sound measurements obtained by quantitative ultrasonography
in axial transmission mode can be used
to precisely measure superficial cortical bone properties
of third metacarpal bone, radius, and tibia in horses.
(Am J Vet Res 2002;63:1464–1469)