Search Results

You are looking at 1 - 2 of 2 items for

  • Author or Editor: Minoru Izumi x
  • Refine by Access: All Content x
Clear All Modify Search

Summary

Serum α1-acid glycoprotein (α1-ag) in bovine fetuses and newborn calves was characterized. Serum α1-ag concentration increased during fetal development and neonatal stages. Mean ± sd serum α1-ag concentration reached a peak of 1,368 ± 207 μg/ml immediately after birth, but thereafter gradually decreased to 249 ± 100 μg/ml, similar to the normal adult bovine range.

By use of isoelectric focusing of thin-layer gels, we detected 7 microheterogeneity bands ranging from pI 3.2 to 3.8 in adult bovine serum. Twelve bands ranging from pI 2.6 to 3.8 were found in 9-month fetuses and in neonates. The 5 most-acidic bands, which are absent in adult serum, ranged between pI 2.6 and 3.1 and decreased with maturation as band patterns assumed adult characteristics.

By crossed affinity electrophoresis, α1-ag of adult bovine serum was separated into 4 peaks according to its differential affinity to concanavalin A (conA). Seventy-five percent of the α1-ag concentration was represented by peak 3 (P-3) and peak 4 (P-4), which had moderate or strong binding to conA. In contrast, fetal sera contained only peak 1 (P-1), which did not have conA-binding affinity. In neonatal sera, 4 peaks were recognized, of which P-1 comprised 70% of the total α1-ag. Thereafter, with aging, percentage of P-3 and P-4 increased as band composition approached the normally expected adult pattern.

Free access
in American Journal of Veterinary Research

Summary

Equine α1-acid glycoprotein (α1 ag) was isolated from equine serum by successive ammonium precipitation, anion- and cation-exchange chromatographies, and gel filtration. Purified equine α1 ag had a molecular weight of 46,000 ± 1,000, and contained 31.4% carbohydrate. Gel isoelectric focusing revealed an isoelectric point range of 2.8 to 3.7. With immunoelectrophoresis, it was found that α1 ag migrated to the α1-globulin region.

Single radial immunodiffusion was used for quantitative measurement of α1 ag in equine serum. In clinically normal foals, serum α1 ag was undetectable (≤ 20 ng/ml) in ≤ 7-day-old foals, but was detected by 14 days. The α1 ag concentration (mean ± sd) increased to reach mean adult values of 99.23 ± 26.90 μg/ml by 1 year of age. The α1 ag concentration in pregnant mares decreased at 2 to 3 months before parturition, then gradually increased until 1 day after parturition, when a brief decrease was observed. The concentration increased again at 2 weeks after foaling, then a decrease was observed, after which the α1 ag concentration increased again by 2 to 4 months after parturition.

The concentration of serum α1 ag quickly rose to peak values 2 to 3 days after castration and jejunojejunostomy in adult horses, returning to baseline values by 14 to 28 days after surgery. The α1 ag was concluded to be an acute-phase reactive protein in horses.

Free access
in American Journal of Veterinary Research