Objective—To evaluate lactoferrin and lysozyme content
in various ocular glands of bison and cattle and in
tears of bison.
Sample Population—Tissues of ocular glands
obtained from 15 bison and 15 cattle and tears collected
from 38 bison.
Procedure—Immunohistochemical analysis was
used to detect lysozyme and lactoferrin in formalin-fixed,
paraffin-embedded sections of the ocular
glands. Protein gel electrophoresis was used to analyze
ocular glands and pooled bison tears by use of a
tris-glycine gel and SDS-PAGE. Western blotting was
used to detect lactoferrin and lysozyme.
Results—Immunohistochemical staining for lactoferrin
was evident in the lacrimal gland and gland of the third
eyelid in cattle and bison and the deep gland of the third
eyelid (Harder's gland) in cattle. Equivocal staining for
lactoferrin was seen for the Harder's gland in bison. An
80-kd band (lactoferrin) was detected via electrophoresis
and western blots in the lacrimal gland and gland of
the third eyelid in cattle and bison, Harder's glands of
cattle, and bison tears. An inconsistent band was seen
in Harder's glands of bison. Lysozyme was not detected
in the lacrimal gland of cattle or bison with the use of
immunohistochemical analysis or western blots.
Western blots of bison tears did not reveal lysozyme.
Conclusion and Clinical Relevance—Distribution
of lactoferrin and a lack of lysozyme are similar in the
lacrimal gland of cattle and bison. Differences in other
tear components may be responsible for variability in
the susceptibility to infectious corneal diseases that
exists between bison and cattle. (Am J Vet Res
OBJECTIVE To evaluate effects of simultaneous intra-articular and IV injection of autologous adipose-derived stromal vascular fraction (SVF) and platelet-rich plasma (PRP) to dogs with osteoarthritis of the hip joints.
PROCEDURES Dogs with osteoarthritis of the hip joints that caused signs of lameness or discomfort were characterized on the basis of results of orthopedic examination, goniometry, lameness score, the Canine Brief Pain Inventory (CBPI), a visual analogue scale, and results obtained by use of a pressure-sensing walkway at week 0 (baseline). Dogs received a simultaneous intraarticular and IV injection of SVF and PRP or a placebo. Dogs were examined again 4, 8, 12, and 24 weeks after injection.
RESULTS CBPI scores were significantly lower for the treatment group at week 24, compared with scores for the control group. Mean visual analogue scale score for the treatment group was significantly higher at week 0 than at weeks 4, 8, or 24. Dogs with baseline peak vertical force (PVF) in the lowest 25th percentile were compared, and the treatment group had a significantly higher PVF than did the control group. After the SVF-PRP injection, fewer dogs in the treated group than in the control group had lameness confirmed during examination.
CONCLUSIONS AND CLINICAL RELEVANCE For dogs with osteoarthritis of the hip joints treated with SVF and PRP, improvements in CBPI and PVF were evident at some time points, compared with results for the control group.