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  • Author or Editor: Mae K. Lopez x
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in Journal of the American Veterinary Medical Association

SUMMARY

Free, autogenous, full-thickness skin grafts were applied to 10 dogs; 5 dogs were given an iron chelator, deferoxamine-10% hydroxyethyl pentafraction starch (def-hes; 50 mg/kg of body weight, iv), and 5 dogs were given an equal volume of 10% hydroxyethyl pentafraction starch (hes) in 0.9% saline solution (5 ml/kg, iv). All dogs (def-hes/hbo- and hes/hbo-treated) were exposed to 60 minutes of hyperbaric oxygen (hbo) at 2 atmospheres absolute pressure twice daily for 10 days, beginning the day of surgery. The percentage of viable graft on day 10 was lower in hes/hbo-treated dogs (mean ± sd, 13.3 ± 21.3%; median, 3.0%) than in def-hes/hbo-treated dogs (64.7 ± 39.2%; 88.3%; P = 0.095, Mann-Whitney two-tailed test). There was a positive correlation between percentage of viable graft (on day 10) and percentage of haired skin on the graft site (on day 28) for all dogs (r = 0.91) and for hes/hbo-treated dogs (r = 0.97). The def-hes/hbo-treated dogs had less consistent correlation (r = 0.67). Perivascular aggregates of foamy cells were observed in the superficial and reticular portions of the dermis and in the subcutaneous tissue on both surfaces of the panniculus muscle in the graft sites of def-hes/hbo-treated dogs. These cells were also observed in the dermis, but not subcutaneous tissue of the control skin sections, and in some viscera of def-hes/hbo-treated dogs. Deferoxamine appears to attenuate the detrimental effect of hbo and hes on survival of free skin grafts. However, clinical use of hbo is not recommended as adjunct treatment for free skin grafts in dogs in the first 10 days after grafting. Administration of def-hes is not recommended because it has failed to improve the survival of free skin grafts, and the consequence of the cellular response seen in this study is undetermined.

Free access
in American Journal of Veterinary Research

SUMMARY

Free, autogenous, full-thickness skin grafting was performed on 10 dogs; 5 dogs were given an iron chelator, deferoxamine-10% hydroxyethyl pentafraction starch (DEF-HES; 50 mg/kg of body weight, IV), and 5 dogs were given 10% hydroxyethyl pcntafrac-tion starch (HES) in 0.9% saline solution (5 ml/kg, iv). The percentage of viable graft on day 10 was higher, but not significantly so, in DEF-HES-treated dogs (mean ± SD, 72.6 ± 24.8%; median 76.5%) than in HES-treated dogs (mean ± SD, 46.7 ± 34.3%; median, 48.8%). A trend for a positive correlation between the percentage of viable graft (on day 10) and the percentage of original graft area (on day 28) was observed in MES- and DEF-HES-treated dogs; this trend was significant in HES-treated dogs (P = 0.012). Both groups had significant positive correlation between percentage of viable graft on day 10 and percentage of haired skin on day 28 (HES, P = 0.000002; DEF-HES. P = 0.0148). A unique finding in DEF-HES treated dogs was the consistent observation of foamy macrophages in the dermis adjacent to the grafts, in deep subcutaneous tissue below the grafts, and in normal dermis.

Free access
in American Journal of Veterinary Research