A 3-year-old domestic hen (Gallus gallus domesticus) was evaluated for acute onset, nonprogressive, non–weight-bearing lameness and paresis of the left pelvic limb. The patient was kept as part of a backyard flock of 7 chickens. There was no history of trauma, and examination by the attending veterinarian prior to referral revealed no external signs of trauma. The owner reported that no abnormalities were observed in terms of the chicken's appetite or defecation.
At the time of referral and hospital admission, the patient had a non–weight-bearing lameness of the left pelvic limb, with minimal motor function, a weak
Objective—To determine whether repeated exposure to clinically relevant concentrations of tricaine methanesulfonate (MS-222) would alter retinal function or induce histologically detectable retinal lesions in koi carp (Cyprinus carpio).
Procedures—2 fish were euthanized at the start of the study, and eyes were submitted for histologic evaluation as untreated controls. Anesthesia was induced in the remaining fish with 200 mg of MS-222/L and maintained with concentrations of 125 to 150 mg/L for a total exposure time of 20 minutes daily on 1 to 13 consecutive days. On days 1, 7, and 13, electroretinography of both eyes was performed in all fish remaining in the study, and 2 fish were euthanized immediately after each procedure for histologic evaluation of the eyes. Median b-wave amplitudes were compared among study days for right eyes and for left eyes via 1-way repeated-measures ANOVA with a Bonferroni correction for multiple comparisons.
Results—Median b-wave amplitudes on days 1, 7, and 13 were 17.7, 20.9, and 17.6 μV, respectively, for right eyes and 15.1, 16.9, and 14.3 μV, respectively, for left eyes. No significant differences in b-wave amplitudes were detected among study days. No histopathologic abnormalities were identified in the retinas of any fish treated with MS-222 or in control fish.
Conclusions and Clinical Relevance—Short-term exposure of koi carp to clinically relevant concentrations of MS-222 daily for up to 13 days was not associated with changes in retinal structure or function as measured in this study.
A 10-year-old 9.4-kg (20.7-lb) neutered male Fox Terrier with atopic dermatitis (originally diagnosed at 16 months of age) triggered by environmental and food allergens underwent reevaluation. Since the time of the initial diagnosis, the dog regularly received immunotherapy injections,a shampoo therapy,b and ear cleaning with a topical ear cleanserc as well as a hypoallergenic prescription dietd to control pruritus. Three years prior, a diagnosis of inflammatory bowel disease had been made, which was also controlled and in remission through consumption of the hypoallergenic prescription diet.d At the time of
Objective—To evaluate a commercially available modified-live Streptococcus equi subsp equi vaccine for safety and persistence in vaccinated ponies and to detect recombination or reversion events in the vaccine strain.
Animals—5 ponies that were 1.5 to 8 years old (group 1) and 4 ponies that were 6 months old (group 2).
Procedures—Ponies were vaccinated, with a subsequent booster vaccination 2 to 3 weeks later, and monitored for 50 days. At booster vaccination, an equal amount of a tetracycline-resistant wild-type strain of S equiwas administered. Recovery of all strains was performed by use of bacteriologic culture and PCR assays.
Results—Ponies in group 1 had background antibody titers against S equi antigen before vaccination despite the lack of known exposure to S equi. Ponies in group 2 were immunologically naïve. Increases in anti-S equi antibody titers were detected in both groups. Ponies in group 1 did not have clinical signs of disease caused by S equi. In group 2, all ponies developed abscesses in retropharyngeal lymph nodes; 1 pony developed severe clinical disease and was euthanized. The vaccine strain was recovered from ponies in group 2 for up to 24 days after vaccination.
Conclusions and Clinical Significance—Although the vaccine was successful in inducing IgG antibodies against S equi in all ponies, findings suggested that the vaccine may have caused substantial morbidity and some deaths in the young ponies. In young ponies, the vaccine strain persisted in tissues for weeks; however, no evidence of recombination was detected.
A 3-year-old 65-kg Nubian doe had a 2-week history of lethargy and weakness that progressed to recumbency. The doe did not improve with treatment of penicillin (20,000 to 40,000 U/kg/d, IM) and meloxicam (0.5 mg/kg, SC, q 36 h) at the onset of the clinical signs. Two weeks before the clinical presentation, the doe had dystocia associated with stillbirth of twins and received fenbendazole (10 mg/kg, PO) just prior to the delivery.
Clinical and Gross Findings
On physical examination, the doe had white oral and ocular mucous membranes and continuous cough and wheezes on thoracic auscultation. The abdomen was
Objective—To describe clinical outcome of dogs with mast cell tumors (MCTs) arising from the oral mucosa, oral mucocutaneous junction, or perioral region of the muzzle and evaluate the potential role of the chemokine receptor type 7 (CCR7) in the biological behavior of these tumors.
Design—Retrospective case series.
Animals—44 dogs with MCTs of the oral mucosa (n = 14), oral mucocutaneous junction (19), or perioral region of the muzzle (11).
Procedures—Medical records were reviewed for information on signalment, regional metastasis, treatments, cause of death, and survival time. Twenty of the 44 cases had stored histologic samples available for immunohistochemical staining for CCR7
Results—For all dogs, median survival time was 52 months. Twenty-six (59%) dogs had regional lymph node metastasis on admission. Median survival time for dogs with lymph node metastasis was 14 months, whereas median survival time was not reached for dogs without lymph node metastasis. Intensity of staining for CCR7 was not significantly associated with the presence of regional lymph node metastasis or survival time.
Conclusions and Clinical Relevance—Results suggested that in dogs with MCTs arising from the oral mucosa, oral mucocutaneous junction, or perioral region of the muzzle, the presence of regional lymph node metastasis at the time of diagnosis was a negative prognostic factor. However, prolonged survival times could be achieved with treatment. In addition, CCR7 expression in the primary tumor was not significantly associated with the presence of regional lymph node metastasis or survival time.