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in Journal of the American Veterinary Medical Association


Objective—To evaluate the use of the anesthetic combination tiletamine, zolazepam, ketamine, and xylazine (TKX) for anesthesia of feral cats at largescale neutering clinics.

Design—Original study.

Animals—7,502 feral cats.

Procedure—Cats were trapped by their caretakers for a feral cat neutering program from July 1996 to August 2000. The anesthetic combination TKX was injected IM into cats while they remained in their traps. Each milliliter of TKX contained 50 mg of tiletamine, 50 mg of zolazepam, 80 mg of ketamine, and 20 mg of xylazine. Females were spayed by veterinarians, whereas males were castrated by veterinarians or veterinary students. Yohimbine (0.5 mg, IV) was administered at the end of the procedure. Logs were kept of the individual drug doses, signalment of the cats, and any complications encountered. These data were analyzed retrospectively (1996 to 1999) and prospectively (2000).

Results—Of the 5,766 cats for which dosing records were complete, 4,584 (79.5%) received a single dose of TKX. The mean initial dose of TKX was 0.24 ± 0.04 ml/cat, and the total mean dose of TKX was 0.27 ± 0.09 ml. Overall mortality rate was 0.35% (26/7,502) cats, and the death rate attributable solely to potential anesthetic deaths was 0.23% (17/7,502) cats.

Conclusions and Clinical Relevance—The use of TKX for large-scale feral cat neutering clinics has several benefits. The TKX combination is inexpensive, provides predictable results, can be administered quickly and easily in a small volume, and is associated with a low mortality rate in feral cats. (J Am Vet Med Assoc 2002; 220:1491–1495)

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in Journal of the American Veterinary Medical Association


Objective—To determine whether the active metabolite of leflunomide, A77 1726 (A77), inhibits replication of feline herpesvirus-1 (FHV-1) in cell culture.

Study Population—Crandell Rees feline kidney (CRFK) cell cultures.

Procedures—Cell cultures were inoculated with FHV-1 and treated simultaneously with concentrations of A77 ranging from 0 to 200μM. The antiviral effect of A77 was determined by use of conventional plaque reduction assays. The effect of A77 on viral load was determined via real-time PCR analysis, and transmission electron microscopy was used to evaluate the effect of A77 on viral morphology. To determine whether the antiviral effect was attributable to alterations in CRFK cell viability and number, CRFK cells were treated with various concentrations of A77 and stained with Annexin V and propidium iodide to assess apoptosis and a mitochondrial function assay was used to determine cell viability.

Results—Concentrations of A77 ≥ 20μM were associated with substantial reduction in plaque number and viral load. Concentrations ≥ 100μM were associated with complete suppression of plaque formation. At low concentrations of A77, clusters of intracytoplasmic virus particles that appeared to lack tegument and an external membrane were detected. Treatment of uninfected CRFK cell monolayers with A77 was associated with reduction in mitochondrial function with minimal evidence of apoptosis.

Conclusions and Clinical Relevance—Leflunomide may be an alternative to current calcineurin-based immunosuppressive protocols used in feline organ transplantation because of its antiherpesviral activity.

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in American Journal of Veterinary Research