Objective—To develop a set of microsatellite markers,
composed of a minimal number of these markers,
suitable for use in forensic genetic investigations
Sample Population—Blood, tissue, or buccal epithelial
cells from 364 dogs of 85 breeds and mixed
breeds and 19 animals from related species in the
Procedure—61 tetranucleotide microsatellite markers
were characterized on the basis of number and size of
alleles, ease of genotyping, chromosomal location, and
ability to be coamplified. The range in allele size, number
of alleles, total heterozygosity, and fixation index for each
marker were determined by use of genotype data from
383 dogs and related species. Polymorphism information
content was calculated for several breeds of dogs.
Results—7 microsatellite markers could be coamplified.
These markers were labeled with fluorescent
dyes, multiplexed into a single reaction, and optimized
for resolution in a commercial genetic analyzer.
The multiplex set was used to identify sires for 2
mixed litters. The test was not species specific; genotype
information collected for wolves, coyotes, jackals,
New Guinea singing dogs, and an African wild
dog could not distinguish between these species.
Conclusions and Clinical Relevance—This set of 7
microsatellite markers is useful in forensic applications
(ie, identification of dogs and determination of
parentage) in closely related animals and is applicable
to a wide range of species belonging to the family
Canidae. (Am J Vet Res 2004;65:1446–1450)
Objective—To assess the heritability of pancreatic
acinar atrophy (PAA) in German Shepherd Dogs
(GSDs) in the United States.
Animals—135 GSDs belonging to 2 multigenerational
Procedure—Two multigenerational pedigrees of
GSDs with family members with PAA were identified.
The clinical history of each GSD enrolled in the study
was recorded, and serum samples for canine trypsinlike
immunoreactivity (cTLI) analysis were collected
from 102 dogs. Dogs with a serum cTLI concentration
≤ 2.0 µg/L were considered to have exocrine pancreatic
insufficiency (EPI) and were assumed to have
Results—Pedigree I consisted of 59 dogs and pedigree
II of 76 dogs. Serum cTLI concentrations were
measured in 48 dogs from pedigree I and 54 dogs
from pedigree II. A total of 19 dogs (14.1%) were
determined to have EPI, 9 in pedigree I (15.3%) and
10 in pedigree II (13.6%). Of the 19 dogs with EPI, 8
were male and 11 were female.
Conclusion and Clinical Relevance—Evaluation
of data by complex segregation analysis is strongly
suggestive of an autosomal recessive mode of
inheritance for EPI in GSDs in the United States.
(Am J Vet Res 2002;63:1429–1434)