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  • Author or Editor: Lance E. Perryman x
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Objective

To determine whether a recently developed test would correctly identify horses heterozygous for the severe combined immunodeficiency (SCID) trait.

Design

Case series.

Animals

17 healthy Arabian horses that had previously produced foals with SCID, 1 healthy Arabian foal whose dam and sire had produced foals with SCID, 4 foals with SCID, and 1 healthy non-Arabian foal.

Procedure

DNA was extracted from leukocytes or fibroblasts, amplified by means of polymerase chain reaction, and hybridized with probes specific for the normal and mutant alleles of the catalytic subunit of DNA-dependent protein kinase, the factor whose absence is responsible for SCID in Arabian foals.

Results

Amplified DNA from the healthy non-Arabian foal hybridized only to the probe specific for the normal allele, whereas amplified DNA from the 4 foals with SCID hybridized only to the probe specific for the mutant allele. Amplified DNA from the 2 stallions and 15 mares hybridized with both probes, as did amplified DNA from the healthy foal whose dam and sire had previously produced foals with SCID, indicating that these horses were all heterozygous for the SCID trait.

Clinical Implications

Results suggest that the genetic test will be useful in identifying Arabian horses heterozygous for the SCID trait and foals with SCID, provided that all Arabian horses with SCID have the same genetic mutation. (J Am Vet Med Assoc 1997;211:1268–1270)

Free access
in Journal of the American Veterinary Medical Association

Summary

Direct effects of equine infectious anemia virus (eiav) on hematopoiesis in vitro were studied. Bone marrow mononuclear cells from clinically normal horses were incubated with 100 TCID50 of ELAV/107 cells. These cells were cultured to assay for colonies derived from erythroid pro-genitors, granulocyte/monocyte progenitors, and fibroblastic progenitors. The eiav had a selective suppressive effect on the erythroid progenitors. Colony-forming units-erythroid were suppressed to 80% of that for medium controls (P = 0.011). Burst-forming units-erythroid were suppressed to 70% of that for medium controls (P = 0.003). Significant effect was not apparent on colony-forming units-granulocyte/macrophage or on colony-forming units-fibroblastic.

Free access
in American Journal of Veterinary Research