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  • Author or Editor: Kendra C. Decile x
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Abstract

Objective—To compare the effects of an orally administered corticosteroid (prednisone), an inhaled corticosteroid (flunisolide), a leukotriene-receptor antagonist (zafirlukast), an antiserotonergic drug (cyproheptadine), and a control substance on the asthmatic phenotype in cats with experimentally induced asthma.

Animals—6 cats with asthma experimentally induced by the use of Bermuda grass allergen (BGA).

Procedures—A randomized, crossover design was used to assess changes in the percentage of eosinophils in bronchoalveolar lavage fluid (BALF); airway hyperresponsiveness; blood lymphocyte phenotype determined by use of flow cytometry; and serum and BALF content of BGA-specific IgE, IgG, and IgA determined by use of ELISAs.

Results—Mean ± SE eosinophil percentages in BALF when cats were administered prednisone (5.0 ± 2.3%) and flunisolide (2.5 ± 1.7%) were significantly lower than for the control treatment (33.7 ± 11.1%). We did not detect significant differences in airway hyperresponsiveness or lymphocyte surface markers among treatments. Content of BGA-specific IgE in serum was significantly lower when cats were treated with prednisone (25.5 ± 5.4%), compared with values for the control treatment (63.6 ± 12.9%); no other significant differences were observed in content of BGA-specific immunoglobulins among treatments.

Conclusions and Clinical Relevance—Orally administered and inhaled corticosteroids decreased eosinophilic inflammation in airways of cats with experimentally induced asthma. Only oral administration of prednisone decreased the content of BGAspecific IgE in serum; no other significant local or systemic immunologic effects were detected among treatments. Inhaled corticosteroids can be considered as an alternate method for decreasing airway inflammation in cats with asthma. (Am J Vet Res 2005;66:1121–1127)

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in American Journal of Veterinary Research

Abstract

Objective—To evaluate changes in cysteinyl leukotriene (LT) concentrations in urine and bronchoalveolar lavage fluid (BALF) in cats with experimentally induced asthma.

Animals—19 cats with experimentally induced asthma and 5 control cats.

Procedure—Cats were sensitized to Bermuda grass or house dust mite allergen, and phenotypic features of asthma were confirmed with intradermal skin testing, evaluation of BALF eosinophil percentages, and pulmonary function testing. A competitive ELISA kit for LTC4, LTD4, and LTE4 was used for quantitative analysis of LTs. Urinary creatinine concentrations and BALF total protein (TP) concentrations were measured, and urinary LT-to-creatinine ratios and BALF LTto- TP ratios were calculated.

Results—Mean urinary LT-to-creatinine ratios did not differ significantly between control cats and allergensensitized cats before or after sensitization and challenge exposure with saline (0.9% NaCl) solution or allergen, respectively. In BALF, the mean LT-to-TP ratio of control cats did not differ significantly before or after sensitization and challenge exposure with saline. Asthmatic cats had BALF LT-to-TP ratios that were significantly lower than control cats at all time points, whereas ratios for asthmatic cats did not differ significantly among the various time points.

Conclusions and Clinical Relevance—Although LTs were readily detectable in urine, no significant increases in urinary LT concentrations were detected after challenge in allergen-sensitized cats. Spot testing of urinary LT concentrations appears to have no clinical benefit for use in monitoring the inflammatory asthmatic state in cats. The possibility that cysteinyl LTs bind effectively to their target receptors in BALF and, thus, decrease free LT concentrations deserves further study. (Am J Vet Res 2003;64:1449–1453)

Full access
in American Journal of Veterinary Research