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Abstract

OBJECTIVE

To evaluate the clinical utility of 3 in-house tests to screen for bacteriuria in dogs with urinary conditions: a computerized urine sediment analyzer (SediVue), a rapid immunoassay (RapidBac), and in-house urine culture. Accuracy of culture and antimicrobial susceptibility (C&S) results from submission of the positive in-house culture plate and a 24- to 48-hour refrigerated urine sample was also assessed.

METHODS

Sterile urine samples from 101 client-owned dogs were used to evaluate sensitivity, specificity, positive predictive value, and negative predictive value of 3 in-house diagnostic tests to detect bacteriuria using C&S testing at a reference laboratory as the reference standard. For positive in-house culture plates, the plate and a 24- to 48-hour refrigerated urine sample were submitted to the reference laboratory for C&S testing and compared to the reference standard.

RESULTS

The reference standard C&S was positive in 25 of 101 cases (25%). All 3 in-house diagnostic tests had high negative predictive values: SediVue was 89% (95% CI, 81% to 96%), RapidBac was 93% (95% CI, 88% to 99%), and in-house culture was 99% (95% CI, 96% to 100%). Positive predictive values were 89% (95% CI, 74% to 100%) for SediVue, 77% (95% CI, 61% to 93%) for RapidBac, and 86% (95% CI, 73% to 99%) for in-house culture. Culture and antimicrobial susceptibility results from submission of the positive in-house plates and refrigerated urine samples approximated the reference standard.

CONCLUSIONS

SediVue, RapidBac, and in-house cultures performed well to rule out canine bacteriuria.

CLINICAL RELEVANCE

Inexpensive in-house diagnostic tests can be used to screen for bacteriuria prior to submission of C&S testing, which promotes antimicrobial stewardship, responsible use of client resources, and access to veterinary care.

Open access
in Journal of the American Veterinary Medical Association
in Journal of the American Veterinary Medical Association