Objective—To determine the effects of intestinal ischemia and reperfusion on the expression of tumor necrosis factor (TNF)-α and interleukin (IL)-6 mRNAs in the jejunum, liver, and lungs of dogs.
Animals—8 healthy adult Beagles.
Procedures—In each dog, the cranial mesenteric artery was occluded for 0 (control group; n = 4) or 60 (I-R group; 4) minutes, followed by reperfusion for 480 minutes; serum TNF-α and IL-6 activities and expression levels of TNF-α and IL-6 mRNAs in jejunal, hepatic, and lung tissues were measured before and at the end of the ischemic period and at intervals during reperfusion. For each variable, values were compared between the control and I-R groups at each time point.
Results—Compared with the control group, serum IL-6 activity increased significantly after 180 minutes of reperfusion in the I-R group; also, jejunal TNF-α mRNA expression increased significantly after 60 (peak) and 180 minutes of reperfusion. In the I-R group, expressions of IL-6 mRNA in the liver and TNF-α and IL-6 mRNAs in the lungs increased significantly at 480 minutes of reperfusion, compared with the control group. Serum TNF-α activity, expression of IL-6 mRNA in the jejunum, and expression of TNF-α mRNA in the liver in the control and I-R groups did not differ.
Conclusions and Clinical Relevance—Results indicated that the liver, lungs, and jejunum contributed to the production of TNF-α and IL-6 after intestinal ischemia and reperfusion in dogs, suggesting that intestinal ischemia and reperfusion induce a systemic proinflammatory cytokine response in dogs.