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  • Author or Editor: Jolene A. Reiter x
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SUMMARY

Chemotaxis under agarose was evaluated to establish an assay system and to characterize chemotactic responses of canine neutrophils. A method for the measurement of canine neutrophil chemotaxis was established, with optimal responses obtained with agarose containing 10% pooled canine serum, a concentration of 5 × 105 cells/well, zymosan-activated serum (zas), or autologous serum or plasma as the chemoattractants, and a 120-minute incubation period. Canine neutrophils responded well to zas, heat-inactivated zas, autologous serum and plasma, and heat-inactivated pooled serum. Chemotactic activity was proportional to the concentration of serum used as the chemoattractant. Mean (± sd) random migration, chemotaxis, chemotactic index, and chemotactic differential of neutrophils from 9 healthy Greyhounds were 1.09 (± 0.23), 1.95 (± 0.38), 1.82 (± 0.31), and 0.86 (± 0.32) mm, respectively.

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in American Journal of Veterinary Research