To evaluate the time and number of laser beam passes required to make full-thickness skin incisions and extent of laser-induced tissue artifacts following use of a CO2 laser at various settings.
24 skin specimens from six 5-month-old porcine carcasses.
4 full-thickness skin specimens were harvested from the flank regions of each carcass within 30 minutes after euthanasia and randomly assigned to 4 treatment groups. Three 5-cm-long incisions were made in each specimen with a CO2 laser (beam diameter, 0.4 mm) set to deliver a continuous wave of energy alone (groups 1 and 2) or in superpulse mode (groups 3 and 4) at 10 (groups 1 and 3) or 20 (groups 2 and 4) W of power. The time and number of passes required to achieve a full-thickness incision were recorded, and extent of laser-induced tissue artifact (as determined by histologic evaluation) was compared among the 4 groups.
Mean time required to make a full-thickness skin incision for groups 2 and 4 (power, 20 W) was significantly less than that for groups 1 and 3 (power, 10 W). Mean number of passes was lowest for group 2 (continuous wave at 20 W). Extent of laser-induced tissue artifact was greatest for group 4 (superpulse mode at 20 W).
CONCLUSIONS AND CLINICAL RELEVANCE
Results provided preliminary information regarding use of CO2 lasers to make skin incisions in veterinary patients. In vivo studies are necessary to evaluate the effect of various CO2 laser settings on tissue healing and patient outcome.
OBJECTIVE To compare the leak pressure and amount of time required to perform closure for 2 double-layer esophagotomy closure techniques.
SAMPLE 28 intrathoracic esophageal segments harvested from 38 porcine cadavers.
PROCEDURES Longitudinal 3-cm esophagotomy incisions made in porcine cadaveric esophagi were closed with 2 double-layer closure techniques. Fifteen incisions were closed with a simple interrupted pattern, and 13 incisions were closed with a simple continuous pattern. Leak pressure, bursting wall tension, and closure time were compared between suture patterns by use of a t test or Mann-Whitney rank sum test.
RESULTS Median leak pressures differed significantly between segments closed with the simple interrupted pattern (16.0 mm Hg; range, 5.4 to 54.9 mm Hg) and the simple continuous pattern (38.7 mm Hg; range, 11.3 to 81.9 mm Hg). Median bursting wall tension differed significantly between the simple interrupted pattern (0.63 × 105 dynes/cm; range, 0.16 × 105 dynes/cm to 2.89 × 105 dynes/cm) and the simple continuous pattern (1.79 × 105 dynes/cm; range, 0.44 × 105 dynes/cm to 4.70 × 105 dynes/cm). Mean ± SD closure time differed significantly between the simple interrupted pattern (19.2 ± 2.0 minutes) and the simple continuous pattern (14.7 ± 1.5 minutes).
CONCLUSIONS AND CLINICAL RELEVANCE In the study reported here, double-layer simple continuous closure resulted in a higher median postoperative leak pressure and higher median postoperative bursting wall tension and could be performed more rapidly than the double-layer simple interrupted closure on these porcine cadaveric specimens.
Objective—To determine the prevalence of detectable serum IgG concentrations in calves prior to ingestion of colostrum and to assess whether a detectable IgG concentration was related to dam parity, calf birth weight, calf sex, season of calving, or infectious agents that can be transmitted transplacentally.
Animals—170 Holstein dairy calves.
Procedures—Serum samples were obtained from calves prior to ingestion of colostrum, and serologic testing for bovine viral diarrhea virus (BVDV) and Neospora caninum was performed. Relative risk, attributable risk, population attributable risk, and population attributable fraction for calves with a detectable serum IgG concentration attributable to positive results for N caninum and BVDV serologic testing were calculated. Logistic regression analysis was used to determine whether dam parity, calf sex, season of calving, and calf weight were associated with precolostral IgG concentration.
Results—90 (52.9%) calves had a detectable total serum IgG concentration (IgG ≥ 16 mg/dL). Relative risk, attributable risk, population attributable risk, and population attributable fraction for calves with a detectable serum IgG concentration attributable to positive results for N caninum serologic testing were 1.66, 0.34, 0.014, and 0.03, respectively. Calf sex, calf birth weight, and season of calving were not significant predictors for detection of serum IgG in precolostral samples.
Conclusions and Clinical Relevance—Prevalence of IgG concentrations in precolostral serum samples was higher than reported elsewhere. There was no apparent link between serum antibodies against common infectious agents that can be transmitted transplacentally and detection of measurable serum IgG concentrations.
OBJECTIVE To determine whether stored (cooled or frozen-thawed) jejunal segments can be used to obtain dependable leak pressure data after enterotomy closure.
SAMPLE 36 jejunal segments from 3 juvenile pigs.
PROCEDURES Jejunal segments were harvested from euthanized pigs and assigned to 1 of 3 treatment groups (n = 12 segments/group) as follows: fresh (used within 4 hours after collection), cooled (stored overnight at 5°C before use), and frozen-thawed (frozen at −12°C for 8 days and thawed at room temperature [23°C] for 1 hour before use). Jejunal segments were suspended and 2-cm enterotomy incisions were made on the antimesenteric border. Enterotomies were closed with a simple continuous suture pattern. Lactated Ringer solution was infused into each segment until failure at the suture line was detected. Leak pressure was measured by use of a digital transducer.
RESULTS Mean ± SD leak pressure for fresh, cooled, and frozen-thawed segments was 68.3 ± 23.7 mm Hg, 55.3 ± 28.1 mm Hg, and 14.4 ± 14.8 mm Hg, respectively. Overall, there were no significant differences in mean leak pressure among pigs, but a significant difference in mean leak pressure was detected among treatment groups. Mean leak pressure was significantly lower for frozen-thawed segments than for fresh or cooled segments, but mean leak pressure did not differ significantly between fresh and cooled segments.
CONCLUSIONS AND CLINICAL RELEVANCE Fresh porcine jejunal segments or segments cooled overnight may be used for determining intestinal leak pressure, but frozen-thawed segments should not be used.
Objective—To evaluate the elimination kinetics of
chlorhexidine in milk when used as an intramammary
infusion to stop lactation in cows.
Procedure—The study was performed in 2 phases.
Three cows were studied in each phase. All cows were
treated with chlorhexidine suspension by infusion into a
mastitic mammary gland quarter after 2 milkings 24
hours apart. Foremilk samples (100 mL) were collected
from treated and untreated (controls) mammary gland
quarters of each cow. Chlorhexidine was extracted from
raw milk, and residue concentrations were quantified by
use of high-performance liquid chromatography.
Foremilk samples from days 2, 5, and 8 were analyzed
in phase I, and samples from time 0 and days 3, 7, 14,
21, 28, 35, and 42 were analyzed in phase II.
Results—In phases I and II, there was no quantifiable
transference of chlorhexidine to milk in untreated
mammary gland quarters. Measurable chlorhexidine
residues were found in milk from treated mammary
gland quarters of 2 cows throughout the 42-day sample
period in phase II. Estimated mean elimination
half-life for chlorhexidine in milk was 11.5 days.
Conclusions and Clinical Relevance—On the basis of
the long elimination half-life of chlorhexidine in milk
from treated mammary gland quarters, the lack of
human dietary exposure data to suggest a food tolerance
for chlorhexidine in food products, and the Food
and Drug Administration's published zero tolerance for
chlorhexidine in uncooked edible calf tissues, we do not
recommend extralabel use of chlorhexidine suspension
as a treatment to stop lactation in mastitic mammary
gland quarters of cows. (J Am Vet Med Assoc 2003;
A 2-day-old 44-kg (97-lb) purebred female Holstein calf was evaluated for weakness, signs of depression, and inability to stand unsupported. Immediately after birth, the calf could stand and was tube fed with approximately 4 L of colostrum. Twenty-four hours after birth, the calf was standing and suckled its daily requirement of milk replacer, but 48 hours after birth, the calf was recumbent and only drank half of its 2-L morning milk replacer feeding. The owner had treated the calf with 100 mg of tulathromycin that morning. On initial examination, rectal temperature (39°C [101.5°F]) and heart rate (120 beats/min) were
Case Description—A 13-year-old llama was examined because of lethargy, inappetence, and syncope.
Clinical Findings—Physical examination revealed muffled heart and lung sounds and peripheral edema. Clinicopathologic abnormalities included lymphopenia, hyperglycemia, prerenal azotemia, mild hyponatremia, mild hypoalbuminemia, and high γ-glutamyltransferase and creatine kinase activities. On ultrasonography, the liver appeared hyperechoic and ascites and pleural effusion were seen. Echocardiography revealed severe dilatation of the right atrium, right ventricle, and pulmonary artery; severe tricuspid regurgitation; and high right ventricular systolic pressure consistent with right-sided heart failure secondary to pulmonary hypertension.
Treatment and Outcome—Treatment with furosemide was attempted, but because of failing health, the llama was euthanized 4 weeks later. Macronodular cirrhosis of the liver, glomerulonephritis, and intimal fibrosis and medial hypertrophy of muscular pulmonary arteries were seen on histologic examination of postmortem specimens.
Clinical Relevance—Findings in this case were similar to those reported for human patients with portopulmonary hypertension secondary to hepatic cirrhosis. Pulmonary hypertension secondary to hepatic disease should be considered in the differential diagnosis of right-sided heart failure.
Case Description—A 5-year-old castrated male Vietnamese pot-bellied pig (Sus scrofa) was evaluated because of anorexia, vomiting, diarrhea, and weight loss.
Clinical Findings—Hypermotile gastrointestinal sounds were noted on abdominal auscultation. An inflammatory leukogram, dehydration, prerenal azotemia, hyponatremia, hypochloremia, hyperproteinemia, hyperglobulinemia, hypomagnesemia, and high γ-glutamyl transpeptidase activity were identified. Transabdominal ultrasonography revealed distended loops of small intestine.
Treatment—IV fluid therapy and analgesic treatment were unsuccessful in the resolution of clinical signs. Exploratory laparotomy revealed an ileocecal intussusception involving the distal portion of the ileum. Distal ileal and cecal bypass were achieved via side-to-side anastomosis of the proximal portion of the ileum and spiral colon with a gastrointestinal anastomosis stapler. Ileal transection or occlusion was not performed. Postoperative complications were minimal, and the pig was clinically normal 15 months after surgery and required no special care or diet.
Clinical Relevance—Distal ileal and cecal bypass without ileal transection have not been described previously in Vietnamese pot-bellied pigs. Anastomosis of the proximal portion of the ileum to the spiral colon without major complications represents a novel, technically simple approach to bypass of the distal portion of the ileum and cecum.