Objective—To determine essential fatty acid concentrations
in plasma and tissue before and after supplementation
with n-3 fatty acids in dogs with atopic dermatitis.
Animals—30 dogs with atopic dermatitis.
Procedure—Dogs received supplemental flaxseed oil
(200 mg/kg/d), eicosapentaenoic acid (EPA;
50 mg/kg/d)-docosahexaenoic acid (DHA;
35 mg/kg/d), or mineral oil as a placebo in a doubleblind,
placebo-controlled, randomized trial. Clinical
scores and plasma and cutaneous concentrations of
linoleic acid, arachidonic acid, α-linolenic acid (α-LLA),
EPA, DHA, prostaglandin E2, and leukotriene B4 were
Results—Total plasma concentrations of α-LLA and
EPA increased and those of arachidonic acid
decreased significantly with administration of EPADHA,
and concentrations of α-LLA increased with
flaxseed oil supplementation; nevertheless, there
was no significant change in the concentrations of
these fatty acids or eicosanoids in the skin. There was
no correlation between clinical scores and plasma or
cutaneous concentrations for any of the measured
fatty acids or eicosanoids.
Conclusion and Clinical Relevance—Results indicated
that at the dose used, neither the concentrations
of fatty acids in skin or plasma nor a decrease in
the production of inflammatory eicosanoids was a
major factor involved in the mechanism of action in
dogs with atopy that responded to fatty acid supplementation.
(Am J Vet Res 2005;66:868–873)