Objective—To determine whether Tritrichomonas foetus infection resides in reproductive tract tissues from cats housed for breeding and for which a high prevalence of colonic T foetus infection has been reported.
Animals—61 purebred cats in 36 catteries undergoing elective ovariohysterectomy or castration and for which reproductive tract tissues, feces, and a reproductive history were obtained.
Procedures—Reproductive tract tissues were examined for T foetus via light microscopy, immunohistochemical analysis, and PCR assay. History of reproductive tract disease was examined to detect statistical associations with identified or reported exposure to colonic T foetus infection.
Results—15 of 61 (25%) cats and 22 of 33 (67%) catteries were identified with active or reported T foetus infection. Light microscopic, immunohistochemical, or molecular evidence of T foetus infection of the reproductive tract was not detected in any cats, including 15 cats with colonic T foetus infection, 29 cats residing in a cattery in which T foetus–infected cats were identified, and 8 cats for which gross or light microscopic evidence of reproductive tract disease was identified. There were no differences in total number of litters, number of litters per breeding, kitten mortality rate, or birth defects between cats or catteries infected with T foetus and those for which T foetus infection was not identified.
Conclusions and Clinical Relevance—No evidence of reproductive tract colonization by T foetus was detected in this study. Accordingly, it is unlikely that reproductive tract infection with T foetus plays an important role in overall disease transmission.
Objective—To determine the optimum reaction conditions and detection limits of PCR assay for identification of Pentatrichomonas hominis in DNA extracted from canine feces.
Sample Population—DNA extracted from feces of 4 dogs with diarrhea from which trichomonads were observed, 81 dogs that had feces submitted to a diagnostic laboratory, and 19 dogs residing in a laboratory animal facility.
Procedures—Optimum reaction conditions and absolute and practical detection limits of 2 P hominis 18S species-specific primer pairs were determined by use of an in vitro cultivated canine isolate of P hominis in the presence and absence of canine feces. The optimized PCR assay was applied to amplification of P hominis 18S rRNA genes from DNA extracted from the feces of dogs.
Results—Under optimized conditions, a primer pair was identified as able to detect as few as 1 P hominis organism/180-mg fecal sample. The PCR assay identified P hominis in diarrheic feces of 4 dogs in which trichomonads were seen by light microscopy. The P hominis genes were not amplified from other fecal samples examined.
Conclusions and Clinical Relevance—Molecular identification of P hominis in feces of 4 dogs with trichomonosis and diarrhea reported here validates the identity of this species in such infections. Sensitive and specific PCR amplification of P hominis 18S rRNA genes from DNA extracted from feces will directly facilitate studies examining pathogenicity of this trichomonad and enable differentiation of P hominis from other known or novel species of trichomonads that may infect the gastrointestinal tract of dogs.
Objective—To determine the efficacy of tinidazole for treatment of cats with experimentally induced Tritrichomonas foetus infection.
Animals—8 specific-pathogen-free kittens.
Procedures—Tinidazole was tested for activity against a feline isolate of T foetus in vitro. Kittens were infected orogastrically with the same isolate and treated or not with tinidazole (30 mg/kg, PO, q 24 h for 14 days). Amoxicillin was administered 28 weeks after completion of tinidazole administration to induce diarrhea. Feces were repeatedly tested for T foetus by use of PCR assay and microbial culture for 33 weeks.
Results—Tinidazole killed T foetus at concentrations ≥ 10 μg/mL in vitro. In experimentally induced infection, tinidazole administered at 30 mg/kg decreased T foetus below the limit of molecular detection in 2 of 4 cats. Recrudescent shedding of T foetus, as elicited by amoxicillin-induced diarrhea, was diminished in cats that received prior treatment with tinidazole.
Conclusions and Clinical Relevance—Although tinidazole decreased the detection of T foetus and treated cats were resistant to later efforts to incite the infection, inability of tinidazole to eradicate infection in many cats poses a serious impediment to the drug’s effectiveness in practice.
Objective—To evaluate the efficacy of and optimize a
commercially available culture system for sensitive
and specific in-clinic culture of Tritrichomonas foetus
from cat feces.
Sample Population—Samples of freshly voided
feces from 117 purebred cats and pure cultures of
T foetus obtained from a cat with chronic diarrhea.
Procedure—Optimal conditions for use of the culture
system, such as quantity of fecal inoculum (0.025 to
0.2 g) and cultivation temperature (25 or 37°C [98.6 or
77.0°F]), were determined. Specificity of the system
was examined by attempted culture of Giardia lamblia
and Pentatrichomonas hominis. Sensitivity of the system
to detect T foetus was determined by inoculation
of culture system pouches with serially diluted T foetus suspensions
with and without feces.
Results—Detection limit of the culture system was 1
and 1,000 T foetus organisms without and with feces
from cats, respectively. Optimal fecal inoculum was
< 0.1 g of feces. At 37°C, cultures yielded positive results
in 24 hours; organisms remained viable for 1 to 6 days,
and bacterial overgrowth was common. At 25°C, cultures
yielded positive results in 1 to 11 days; organisms were
long-lived, and bacterial overgrowth was uncommon.
Neither G lamblia or P hominis survived in the culture system.
Conclusions and Clinical Relevance—The culture
system was sensitive and specific for culture of
T foetus in feces of cats. Performance was optimal
when test kits were inoculated with ≤ 0.1 g of freshly
voided feces and cultured at 25°C. (J Am Vet Med Assoc
Objective—To develop an endoscopic technique for
use in monitoring devlopment of gastric ulcers via a
gastric cannula during withholding of feed and administration
of a finely ground diet to pigs.
Animals—6 pigs weighing between 60 and 70 kg.
Procedure—A gastric cannula was surgically inserted
adjacent to the pars esophagea in each pig. Pigs were
fed a finely ground diet for two 7-day periods that were
separated by a 48-hour period during which feed was
withheld. Endoscopic examination via the gastric cannula
was used to monitor development of ulcers in the
pars esophageal region of the pigs during the 48-hour
period of feed withhold and subsequent 7-day feeding
period. An ulcer score was assigned during each endoscopic
examination. A final examination was performed
during necropsy and compared with results for the final
Results—Consumption of a finely ground diet for 7
days resulted in progressive erosive damage to the
pars esophageal region of the stomach. Further significant
increases in ulcerative damage were detected
after 24 and 48 hours of withholding of feed. Final
examination during necropsy did not reveal significant
differences from results obtained during the final
Conclusions and Clinical Relevance—Endoscopic
examination via a gastric cannula was an effective
means of monitoring ulcer development in the pars
esophagea of pigs. Feeding a finely ground diet and
withholding of feed induced endoscopically observable
ulcers in the stratified squamous epithelial region
of the stomach. Direct visual examination during
necropsy confirmed the accuracy of endoscopic
examination. (Am J Vet Res 2002;63:1076–1082)
Objective—To determine whether infection with
Tritrichomonas foetus causes diarrhea in specific pathogen-free or Cryptosporidium coinfected cats.
Animals—4 cats with subclinical cryptosporidiosis
(group 1) and 4 specific-pathogen-free cats (group 2).
Procedure—Cats were infected orogastrically with an
axenic culture of T foetus isolated from a kitten with
diarrhea. Direct microscopy and protozoal culture of
feces, fecal character, serial colonic mucosal biopsy
specimens, and response to treatment with nitazoxanide
(NTZ; group 1) or prednisolone (groups 1 and 2)
Results—Infection with T foetus persisted in all cats
for the entire 203-day study and resulted in diarrhea
that resolved after 7 weeks. Group-1 cats had an earlier
onset, more severe diarrhea, and increased number
of trichomonads on direct fecal examination, compared
with group-2 cats. Use of NTZ eliminated shedding
of T foetus and Cryptosporidium oocysts, but
diarrhea consisting of trichomonad-containing feces
recurred when treatment was discontinued.
Prednisolone did not have an effect on infection with
T foetus but resulted in reappearance of
Cryptosporidium oocysts in the feces of 2 of 4 cats.
During necropsy, T foetus was isolated from contents
of the ileum, cecum, and colon. Tritrichomonas foetus
organisms and antigen were detected on surface
epithelia and within superficial detritus of the cecal
and colonic mucosa.
Conclusions and Clinical Relevance—After experimental
inoculation in cats, T foetus organisms colonize
the ileum, cecum, and colon, reside in close contact
with the epithelium, and are associated with transient
diarrhea that is exacerbated by coexisting cryptosporidiosis
but not treatment with prednisolone.
(Am J Vet Res 2001;62:1690–1697)
Objective—To determine the long-term outcome of
cats infected with Tritrichomonas foetus and identify
treatment and management strategies influencing
resolution of infection or associated diarrhea.
Sample Population—26 cats with T foetus-associated
diarrhea at least 22 months prior to the study.
Procedure—A standardized survey regarding clinical
course and management was administered to owners
of cats with T foetus infection and associated diarrhea.
Fecal samples were obtained from each cat; the
presence of T foetus was assessed via microscopic
examination of smears, culture in commercial media,
and polymerase chain reaction amplification of T foetus rDNA
involving species-specific primers.
Results—Survey responses were obtained from owners
of all 26 cats. Twenty-three cats had complete resolution
of diarrhea a median of 9 months after onset.
Analysis of fecal samples obtained from 22 cats
revealed persistent T foetus infection in 12, with a
median of 39 months after resolution of diarrhea.
History of implementation of a dietary change, treatment
with paromomycin, or higher numbers of cats in
the household was associated with significantly longer
duration of time to resolution of diarrhea.
Conclusions and Clinical Relevance—Results suggested
chronic T foetus-associated diarrhea in most
cats is likely to resolve spontaneously within 2 years
of onset. Chronic infection with T foetus(without clinical
signs) after resolution of diarrhea appears to be
common. Although often temporarily effective in
decreasing severity of diarrhea, attempts to treat cats
with T foetus infection may result in prolongation of
time to resolution of diarrhea. (J Am Vet Med Assoc