Objective—To identify erythrocyte-bound immunoglobulin (Ig) isotypes in dogs with primary immune-mediated hemolytic anemia (IMHA).
Design—Retrospective case series.
Animals—54 dogs with IMHA.
Procedures—Medical records of dogs with IMHA diagnosed between January 2001 and April 2010 were examined. Immunoglobulin isotype (tested via direct immunofluorescence by flow cytometry to identify erythrocyte-bound Ig), Hct, serum bilirubin concentration, presence of autoagglutination, degree of spherocytosis, duration of hospitalization, and 90-day outcome were recorded.
Results—The Hct on admission was significantly lower in dogs with IgG and IgM isotypes bound to erythrocytes, compared with dogs with a single Ig isotype, and the degree of spherocytosis was greater in dogs with IgG and IgM bound to erythrocytes, compared with dogs that only had IgM. Dogs with only IgM were not more likely to have autoagglutination, compared with dogs that only had IgG on the erythrocyte surface. Although Ig isotype was not associated with survival time, initial serum total bilirubin concentration was higher in nonsurvivors.
Conclusions and Clinical Relevance—Results suggested that dogs with IMHA with ≥ 2 Ig isotypes bound to erythrocytes, particularly IgG and IgM, are likely to have a more severe degree of anemia, spherocytosis, and autoagglutination.
Objective—To evaluate the occurrence of perianesthetic complications in dogs undergoing MRI for suspected intracranial disease and identify risk factors associated with observed complications.
Design—Retrospective case-control study.
Animals—238 client-owned dogs undergoing MRI of the brain.
Procedures—Signalment, clinical signs, neurologic examination findings, presumptive diagnosis, anesthesia-related variables, whether CSF was collected and CSF analysis results, severe perianesthetic complications (need for a ventilator following anesthesia or perianesthetic death), and anesthetic recovery time were recorded. Selected factors were compared between dogs with and without intracranial lesions and dogs with and without perianesthetic complications (including severe complications and prolonged anesthetic recovery [> 20 minutes from the end of anesthesia to extubation]).
Results—3 of 149 (2%) dogs with and 0 of 89 dogs without intracranial lesions required ventilation following anesthesia; the difference was nonsignificant. Recovery time was significantly longer in dogs with (median, 15 minutes) than in dogs without (10 minutes) intracranial lesions. Abnormal mentation prior to anesthesia was the only clinical sign that differed significantly between dogs with (15/26 [58%]) and without (70/212 [33%]) perianesthetic complications. A significantly larger proportion of dogs with perianesthetic complications had intracranial masses (13/26 [50%]), compared with dogs without these complications (56/212 [26%]).
Conclusions and Clinical Relevance—Dogs with complications were more likely to have had intracranial lesions than were dogs without complications, but few dogs had severe complications. Abnormal mentation was more common in dogs with than in dogs without complications. Prospective studies to further evaluate perianesthetic risk factors and procedures for improving outcomes in these patients are warranted.