Objective—To determine whether a polymerase
chain reaction (PCR) assay could be used to detect
Eperythrozoon wenyoniin the blood of cattle.
Animals—95 cattle from various herds in Alabama
and Georgia and 96 bulls enrolled in Auburn
University's Alabama Beef Cattle Improvement
Association Bull Test program.
Procedure—Blood samples were collected by means
of venipuncture of the median caudal vein and submitted
for a CBC and PCR assay. Blood smears were
made immediately after blood collection and examined
by means of light microscopy.
Results—Three of 95 cattle from herds in Alabama
and Georgia and 5 of 96 bulls enrolled in the Bull Test
program had positive PCR assay results. Organisms
were seen in blood smears from only 5 of these 8 animals.
Organisms were not seen in blood smears from
any animals for which results of the PCR assay were
Conclusions and Clinical Relevance—Results suggest
that a PCR assay may be an effective method for
detecting E wenyoni infection in cattle and that the
PCR assay may be a more sensitive test than evaluation
of blood smears. (J Am Vet Med Assoc 2001;