Objective—To evaluate the use of a human bladder
tumor antigen test for diagnosis of lower urinary tract
malignancies in dogs.
Sample Population—Urine samples from dogs without
urinary tract abnormalities (n = 18) and from dogs
with lower urinary tract neoplasia (20) or nonmalignant
urinary tract disease (16).
Procedure—Test results were compared among
groups and among 3 observers. The effects of urine
pH and specific gravity, degree of hematuria, and storage
temperature and time of urine samples on test
results were also assessed.
Results—Test sensitivity and specificity were 90 and
94.4%, respectively, for differentiating dogs with
lower urinary tract neoplasia from dogs without
abnormalities. However, specificity decreased to
35% for differentiating dogs with neoplasia from
dogs with nonmalignant urinary tract disease. In
dogs with neoplasia, results were significantly affected
by degree of hematuria. However, addition of
blood to urine from dogs without hematuria had no
significant effect on test results. Although intraobserver
variation was significant, urine pH, specific
gravity, or storage time or temperature had no significant
effect on results.
Conclusions and Clinical Relevance—Although this
bladder tumor antigen test was sensitive for differentiating
dogs with malignancies of the lower urinary
tract from dogs without urinary tract disease, it was
not specific for differentiating dogs with neoplasia
from dogs with other lower urinary tract abnormalities.
It cannot, therefore, be recommended as a definitive
diagnostic aid for the detection of lower urinary
tract malignancies in dogs. (Am J Vet Res