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  • Author or Editor: Ingrid Österlundh x
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Abstract

Objective—To determine the effects of porcine mammary secretions on polymorphonuclear (PMN) leukocyte function and to relate concentrations of estradiol-17β and cortisol in mammary secretions to PMN cell function.

Sample Population—Mammary secretions from 10 healthy sows and blood PMN leukocytes from 27 healthy sows.

Procedure—Mammary secretions were collected within 24 hours after parturition (colostrum) and 12 to 13 days later (milk). Chemoattractant properties were assessed by use of a cell migration assay. Phagocytic capacity of PMN cells in colostrum and milk was assessed by recording chemiluminescence following phagocytosis of Escherichia coli or zymosan. Estradiol-17β and cortisol concentrations were determined by use of radioimmunoassays.

Results—Chemoattractant properties of colostrum and milk were significantly greater than that of zymosan-activated serum. However, chemoattractant properties did not differ significantly between the 2 types of secretions. The capacity of PMN cells in colostrum to phagocytose either zymosan or E coli was less, compared with cells in milk, and the ability of cells in either type of mammary secretion to phagocytose E coli was greater than the ability to phagocytose zymosan. Concentrations of estradiol-17β and cortisol were greater in colostrum, compared with milk. No clear relation was evident between PMN cell activity and hormone concentrations in mammary secretions.

Conclusions and Clinical Relevance—Although chemoattractant properties of colostrum and milk did not differ, the phagocytic capacity of PMN cells in colostrum was significantly less than that of cells in milk. This may predispose sows to coliform mastitis during the early postparturient period. (Am J Vet Res 2001;62:1250–1254)

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in American Journal of Veterinary Research

Abstract

Objective—To determine whether concentrations of proinflammatory cytokines, acute-phase proteins, and cortisol differ at parturition among 3 categories of sows (noninoculated, clinically affected and nonaffected following intramammary inoculation with Escherichia coli).

Animals—16 sows.

Procedure—Sows were allocated to inoculated (n = 12) or noninoculated (4) groups. Inoculated sows received intramammary administration of E coli (serotype O127) during the 24-hour period preceding parturition. Blood samples were collected from noninoculated and inoculated sows for 3 consecutive days within 3 to 11 days before farrowing and inoculation. Samples were also collected 0, 24, 48, 72, and 96 hours after farrowing and inoculation. Inoculated sows were further categorized as affected (4 sows) or nonaffected (8 sows) based on clinical signs of disease. Serum tumor necrosis factor (TNF)-α, plasma interleukin (IL)-6, and serum amyloid A (SAA) concentrations were measured by use of ELISA; serum haptoglobin concentration was assayed by use of a hemoglobin- binding method; and plasma cortisol concentration was determined by use of radioimmunoassay.

Results—Plasma or serum concentrations of TNF-α, IL-6, and SAA of both categories of inoculated sows were significantly increased by 24 hours after intramammary inoculation of E coli, compared with concentrations in noninoculated sows. Concentrations of serum TNF-α and plasma IL-6 were significantly higher in inoculated sows that developed clinical mastitis than in nonaffected inoculated sows.

Conclusions and Clinical Relevance—Concentrations of TNF-α and IL-6 are promising markers for the identification of periparturient sows with subclinical coliform mastitis. Identification of such sows should help improve the health and survival of piglets. (Am J Vet Res 2004;65:1434–1439)

Full access
in American Journal of Veterinary Research