Objective—To determine accuracy and precision of a
point-of-care hemoglobinometer for measuring hemoglobin
concentration and estimating PCV in horses.
Procedure—Blood samples were obtained from 43
horses examined at a veterinary teaching hospital.
Hemoglobin concentration was measured with the
hemoglobinometer and by means of the standard
cyanmethemoglobin method; PCV was measured by
centrifugation. Blood samples were also obtained
from 12 healthy horses, and PCV of aliquots of these
samples was altered to approximately 5 to 80% by
removing or adding plasma. Hemoglobin concentration
and PCV were then measured.
Results—For samples from the clinic patients, hemoglobin
concentrations obtained with the hemoglobinometer
were less than concentrations obtained with
the cyanmethemoglobin method; however, there was
a linear relationship between concentrations obtained
with the 2 methods. Breed, sex, body weight, and
duration of sample storage did not significantly affect
the difference between hemoglobin concentrations
obtained with the 2 methods. There was a significant
linear relationship between PCV and hemoglobinometer
hemoglobin concentration (PCV = [2.83 X
hemoglobin concentration] − 0.62). For samples from
the healthy horses, a substantial negative bias was
evident with the hemoglobinometer when hemoglobin
concentration exceeded 16 g/dL.
Conclusions and Clinical Relevance—Results suggest
that this hemoglobinometer is reasonably accurate
and precise when used to measure hemoglobin
concentration in blood samples from horses with a
hemoglobin concentration < 16 g/dL. (J Am Vet Med