Objective—To compare diagnostic quality of percutaneous
kidney biopsy specimens obtained with
laparoscopy versus ultrasound guidance in dogs and
compare diagnostic quality of specimens obtained
with 14- versus 18-gauge biopsy needles.
Animals—10 healthy dogs.
Procedure—In each dog, 2 biopsy specimens were
obtained from each kidney, 1 with a 14-gauge biopsy
needle and 1 with an 18-gauge biopsy needle. Biopsy
specimens were obtained from 1 kidney by means of
ultrasound guidance and from the contralateral kidney
by means of direct viewing during laparoscopy.
Number of glomeruli, quality of the biopsy specimen,
proportion of specimens that contained muscle tissue,
and proportion of specimens with fragmentation
or crushing were determined.
Results—Mean ± SD number of glomeruli (32.6
± 11.0) in laparoscopic, 14-gauge biopsy specimens
was significantly higher than mean number of
glomeruli in ultrasound-guided, 14-gauge specimens;
mean number of glomeruli in ultrasound-guided, 18-gauge specimens;
and mean number of glomeruli in
laparoscopic, 18-gauge specimens. All 10 laparoscopic,
14-gauge biopsy specimens were classified as
excellent. The proportion of 18-gauge biopsy specimens
with crushing or fragmentation was significantly
higher than the proportion of 14-gauge specimens.
One of the kidneys biopsied with ultrasound guidance
had a large amount of hemorrhage. Hemorrhage was
modest and transient following laparoscopic biopsy.
Conclusions and Clinical Relevance—Results suggest
that excellent-quality renal biopsy specimens
with large numbers of glomeruli can be obtained with
14-gauge, double-spring-activated biopsy needles during
laparoscopy. Renal biopsy specimens obtained
with 18-gauge biopsy needles frequently had few
glomeruli and often were crushed or fragmented,
increasing the difficulty in making an accurate diagnosis.
(J Am Vet Med Assoc 2003;223:317–321)