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  • Author or Editor: Gerhard Wess x
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Abstract

Objective—To evaluate the clinical and analytic accuracy of 5 portable blood glucose meters (PBGM) in cats, with emphasis on the detection of potential sources of error.

Animals—200 cats.

Procedure—Venous blood glucose readings from 5 PBGM were compared with the results of a hexokinase reference method. Agreement among methods was determined by error grid analysis and statistical methods.

Results—A total of 2,975 PBGM readings and 513 reference values were analyzed. The accuracy of the PBGM varied in different glycemic ranges. The largest differences between PBGM readings and reference values were in the high glycemic range; 4 PBGM underestimated and 1 PBGM overestimated the reference values in most instances. In the low and reference glycemic ranges, the absolute differences between PBGM readings and reference values were small. Despite the analytic differences in accuracy, 4 PBGM had 100% and 1 PBGM had 98.7% of readings in the clinically acceptable values of the error grid analysis. Within- and between-day precisions were good for all PBGM. Significant differences were not detected between readings of EDTA and lithium-heparinized blood and fresh blood without anticoagulant. Compared with these blood types, 1 PBGM had significantly different readings with fluoride anticoagulated blood. In blood samples with a low Hct, all PBGM overestimated glucose concentrations. Sample volumes < 3 µl resulted in inaccurate measurements in 3 PBGM.

Conclusions and Clinical Relevance—Performance varied among the 5 PBGM analyzed; however, all PBGM were deemed acceptable for clinical use in cats. (Am J Vet Res 2000;61:1587–1592)

Full access
in American Journal of Veterinary Research

Abstract

Objective—To evaluate clinical and analytical accuracy of 5 portable blood glucose meters (PBGM) used to measure blood glucose concentrations in dogs and to determine potential sources of error.

Design—Prospective study.

Animals—221 dogs.

Procedure—Venous blood samples were obtained, and results of the 5 PBGM were compared with results of a hexokinase reference method. Agreement among methods was determined by use of error grid analysis and statistical methods.

Results—Accuracy of the PBGM varied with glucose concentration of the sample. The largest differences between results of the PBGM and results of the reference method were obtained with samples with high glucose concentrations; 4 PBGM tended to underestimate and 1 PBGM tended to overestimate the true glucose concentration. Absolute differences between results of the PBGM and results of the reference method were small for samples with low glucose concentrations and samples with concentrations in the reference range. None of the PBGM yielded measurements that would result in clinically unacceptable errors. Within-run and between-day precision was good for all PBGM, and results were not affected by use of EDTA or heparin to anticoagulate blood. Readings of the PBGM were significantly higher for blood samples with low Hct than for samples with normal Hct. For 3 PBGM, samples < 3 μl resulted in inaccurate measurements.

Conclusions and Clinical Relevance—Results suggest that currently available PBGM are sufficiently accurate for use in clinical practice to determine blood glucose concentrations in dogs. ( J Am Vet Med Assoc 2000;216:203–209)

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in Journal of the American Veterinary Medical Association

Abstract

Objective—To evaluate the diagnostic value of plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) concentrations in Doberman Pinschers in various stages of dilated cardiomyopathy (DCM).

Animals—328 Doberman Pinschers.

Procedures—Staging of DCM was determined via analysis of results of physical examinations, 24-hour ambulatory ECG (Holter) recordings, and echocardiographic evaluations. Plasma samples for NT-proBNP assays were obtained at each examination. Concentrations of NT-proBNP were measured in 337 samples obtained from 196 healthy Doberman Pinschers (control dogs) and in 195 samples obtained from 132 Doberman Pinschers in various stages of DCM. These included dogs that had ventricular premature contractions (VPCs; 79 samples), echocardiographic changes (23 samples), or both (51 samples); 16 samples were from dogs with overt DCM, and 26 were from dogs that were considered normal during initial examination but developed DCM within 1.5 years after this assessment. Receiver operating characteristic curves were analyzed to determine sensitivity and specificity of NT-proBNP concentrations for detection of DCM.

Results—NT-proBNP concentrations in dogs that had or developed DCM were significantly higher than those of control dogs. Sensitivity and specificity of NT-proBNP concentrations (cutoff value, > 400 pmol/L) to detect all stages of DCM were 81.1 % and 75.0%, respectively; sensitivity was 90.0% and specificity was 75.0% to predict echocardiographic changes. Specificity to detect echocardiographic changes was 90.4% at a cutoff value of 550 pmol/L.

Conclusions and Clinical Relevance—Plasma concentrations of NT-proBNP were increased in dogs with DCM and in apparently healthy dogs that developed DCM within 1.5 years after samples were obtained, compared with concentrations in control dogs.

Full access
in American Journal of Veterinary Research