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  • Author or Editor: Fiona M. Cunningham x
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SUMMARY

Pharmacokinetic and pharmacodynamic variables of flunixin were studied in calves after IV administration of the drug at a dose rate of 2.2 mg/kg of body weight. The anti-inflammatory properties of flunixin were investigated, using a model of acute inflammation; this involved surgically implanting tissue cages at subcutaneous sites and stimulating the tissue cage granulation tissue by intracavitary injection of carrageenan. The actions of flunixin on exudate concentrations of several substances related to the inflammatory process, including proteases (metalloprotease [active and total] and cysteine and serine proteases), enzymes (lactate dehydrogenase, acid phosphatase, and β-glucuronidase [β-glu]), eicosanoid (prostaglandin E2 [pge 2], leukotriene B4, and serum thromboxane B2 [txb 2]) concentrations, and bradykinin (BK)-induced edema, were investigated.

Flunixin had a long elimination half-life—6.87 ± 0.49 hours—and volume of distribution was 2.11 ± 0.37 L/kg, indicating extensive distribution of the drug in the body. Body clearance was 0.20 ± 0.03 L/ kg/h.

Flunixin exerted inhibitory effects on serum txb 2 and exudate pge 2 concentrations, β-glu activity, and BK-induced swelling. Other enzymes and inflammatory mediators were not significantly affected. Pharmacokinetic/pharmacodynamic modeling of the data revealed similar mean concentration producing 50% of the maximal effect values for inhibition of exudate pge 2 and p-glu and of BK-induced swelling (0.070 ± 0.006, 0.064 ± 0.040, and 0.061 ± 0.030 μg/ml, respectively). A lower concentration producing 50% of the maximal effect value was obtained for inhibition of serum txb 2 concentration (0.023 ± 0.004 μg/ml). Differences also were observed in equilibration halflife for these actions, suggesting the existence of 3 distribution compartments correlating with 3 sites of action—a central compartment and shallow and deep peripheral compartments. Pharmacokinetic/pharma- codynamic modeling proved to be a useful analytical method, providing a quantitative description of in vivo drug pharmacodynamics and indicating possible mechanisms of action.

Free access
in American Journal of Veterinary Research

Abstract

Objective—To determine the phosphodiesterase (PDE) isoenzymes in equine platelets and evaluate their influence on platelet adhesion.

Sample Population—Platelets obtained from healthy New Forest Pony geldings that ranged from 12 to 20 years of age (mean ± SEM, 17.3 ± 1.1 years).

Procedures—PDE isoenzyme activity in equine platelets was determined by use of a 2-step radioactive assay. Functional importance of PDE isoenzymes was established by use of selective inhibitors in a colorimetric adhesion assay.

Results—PDE1, PDE2, PDE3, and PDE5 and small amounts of PDE4 were found in equine platelets. Inhibition of PDE3 abolished platelet adhesion almost completely, whereas inhibition of PDE4 and PDE5 had little effect.

Conclusions and Clinical Relevance—Function of equine platelets can be influenced by inhibition of PDE3. Selective PDE3 inhibitors may be clinically useful to regulate platelet function. They offer the advantage of increased potency with fewer adverse effects, compared with those for nonselective PDE inhibitors.

Full access
in American Journal of Veterinary Research

Abstract

Objective—To evaluate the roles of 5-hydroxytryptamine (5-HT), thromboxane A2 (T×A2), and platelet-activating factor (PAF) in endotoxin-induced digital hypoperfusion in horses.

Animals—6 healthy adult Thoroughbreds.

Procedures—Horses were treated with IV administration of saline (0.9% NaCl) solution (control treatment) or the 5-HT1B/D selective antagonist, GR55562 (0.3 mg/kg), prior to tryptamine infusion (1.6 μg/kg/min for 30 minutes) to establish an effective GR55562 dose. In a crossover study, horses were treated with IV administration of saline solution (control treatment), aspirin (4 mg/kg, 2 hours or 4 days before lipopolysaccharide [LPS] infusion), GR55562 (0.3 mg/kg), the PAF antagonist WEB2086 (3 mg/kg), or aspirin plus GR55562 prior to LPS infusion (30 ng/kg for 30 minutes). Digital blood flow was measured by use of Doppler ultrasonography. Concomitant measurements of hoof wall and coronary band surface temperatures were made. Serial blood samples were collected and plasma 5-HT and T×A2 concentrations determined.

Results—GR55562 abolished tryptamine-induced digital hypoperfusion. Neither WEB2086 nor GR55562 affected LPS-induced alterations in digital perfusion or plasma mediator concentrations. Aspirin given 2 hours before LPS administration abolished the increase in plasma T×A2 concentration and significantly attenuated LPS-induced digital hypoperfusion. Aspirin given 4 days before LPS significantly attenuated the increase in plasma T×A2 concentration and digital hypothermia. Aspirin plus GR55562 had a greater effect on LPS-induced digital hypothermia than aspirin alone.

Conclusions and Clinical Relevance—Thromboxane A2 and 5-HT played a role in mediating LPS-induced digital hypoperfusion in horses. Platelet-activating factor appeared unimportant in mediating LPS-induced 5-HT or T×A2 release or digital hypoperfusion.

Full access
in American Journal of Veterinary Research

Abstract

Objective—To determine whether expression of equine platelet activation–dependent surface markers is influenced by phospodiesterase (PDE) isoenzyme activity and whether antigen challenge alters platelet PDE activity in horses with recurrent airway obstruction (RAO).

Animals—16 horses.

Procedures—7 healthy horses were used for in vitro experiments, 6 horses with RAO were used for antigen challenge, and 6 healthy horses were used as control animals. Three of the healthy horses had also been used in the in vitro experiments. Effects of PDE inhibition and activation of adenylyl cyclase on CD41/61 and CD62P expression on platelets and platelet-neutrophil aggregate formation in vitro were investigated via flow cytometry. Platelet PDE activity and sensitivity to inhibition of PDE3 and PDE5 isoenzymes were examined in horses with RAO and control horses before and after antigen challenge.

Results—Inhibition of PDE or activation of adenylyl cyclase significantly inhibited stimulus-induced expression of CD41/61 and CD62P (by approx 94% and 40%, respectively) and percentage of CD62P positive cells (by approx 30%). Only the PDE3 inhibitor, trequinsin, caused a significant (53%) reduction in platelet-neutrophil aggregate formation. Platelet PDE activity decreased following antigen challenge in RAO-affected horses and control horses. In horses with RAO, a significant increase in sensitivity of platelet PDE to inhibition by the PDE5 inhibitor zaprinast was observed after 5 hours.

Conclusions and Clinical Relevance—Results provided further evidence that PDE3 is an important regulator of equine platelet activation and suggested that changes in regulation of platelet PDE5 may contribute to antigen-induced response in horses with RAO.

Full access
in American Journal of Veterinary Research