Objective—To determine effects of intraincisional
bioactive glass on healing of sutured skin wounds in
Animals—9 purpose-bred mature female Beagles.
Procedure—3 small matched bilateral (treated vs
control) full-thickness truncal skin incisions were
made and sutured. Treated wounds received intraincisional
particulate bioactive glass prior to closure.
Laser Doppler perfusion imaging was used to assess
percentage change in tissue perfusion 3 and 5 days
after incision on 1 set of 2 matched wounds, and skin
and subcutaneous tissue-cutaneous trunci breaking
strength were assessed at 5 days. The other 2 sets of
wounds were used for histologic evaluation at 5 and
21 days, respectively.
Results—Subjective signs of gross inflammatory
reaction were not detected in treated or control
wounds. At 5 days, median subcutaneous tissuecutaneous
trunci breaking strength was significantly
higher in treated wounds than in control wounds
(188.75 vs 75.00 g). At 5 days, median scores were
significantly higher for neutrophils (1 vs 0),
macrophages (2 vs 1), and necrosis (1 vs 0) for treated
wounds than for control wounds. At 21 days,
median macrophage scores were significantly higher
for treated wounds than for control wounds (2
Conclusions and Clinical Relevance—Bioactive
glass in soft tissues does not cause a gross inflammatory
reaction but causes an increase in histologic
signs of inflammation, which decreases with time.
Bioactive glass has potential for increasing tissue
strength. Increased subcutaneous breaking strength
could be beneficial in treating wounds in which early
healing strength is needed. (Am J Vet Res 2001;62:1149–1153)
Objective—To determine the effects of a hydrolyzed
bovine collagen dressing (HBCD) on healing of open
wounds in healthy dogs.
Animals—9 female Beagles.
Procedures—2 full-thickness skin wounds were
made bilaterally on the trunk of each dog. Wounds on
1 side were treated with powdered HBCD covered
with a semiocclusive nonadherent bandage. Wounds
on the other side (control wounds) were covered with
a semiocclusive nonadherent bandage only. Wound
healing was subjectively assessed, and percentage
increase in tissue perfusion was assessed by use of
laser Doppler perfusion imaging (LDPI). Planimetry
was performed to determine the percentages of contraction,
epithelialization, and total wound healing.
Biopsy specimens were examined microscopically to
evaluate histologic changes.
Results—The HBCD did not induce a strong inflammatory
reaction, as reflected by results of LDPI and
histologic examination. Moreover, HBCD appeared
hydrophilic and provided an environment to keep
wounds clean and enhance early epithelialization.
After treatment for 7 days, treated wounds had a significantly
greater percentage of epithelialization than
control wounds (12.13 vs 7.03%).
Conclusion and Clinical Relevance—The hydrophilic
property of HBCD may cleanse contaminated
wounds with the body's homeostatic fluids and
enhance early wound epithelialization. (Am J Vet Res
Case Description—A 7-year-old 509-kg (1,120-lb) Tennessee Walking Horse mare was evaluated because of bilateral mucosanguinous nasal discharge, intermittent right-sided epistaxis, and worsening dyspnea of 9 months' duration.
Clinical Findings—Multiple masses in the nasopharynx were detected via endoscopic and radiographic examinations. Cytologic and histologic examinations of biopsy specimens of 1 mass revealed round yeasts with thick nonstaining capsules and occasional narrow-based budding that resembled cryptococcal organisms.
Treatment and Outcome—Oral administration of fluconazole and organic ethylenediamine dihydriodide and intermittent intralesional injections with fluconazole, amphotericin B, and formalin resulted in resolution of lesions for a period of 2.5 years. The horse then developed exophthalmos, recurring clinical signs, and extensive nasopharyngeal masses. The masses were surgically debulked via a large frontonasal bone flap, and the horse was treated with IV injections of amphotericin B and long-term oral administration of fluconazole. Clinical signs did not recur in the following 2-year period. A presumptive diagnosis of cryptococcosis was made following cytologic and histologic evaluations of the masses; results of serologic analysis and fungal culture confirmed infection with Cryptococcus neoformans.
Clinical Relevance—Cryptococcal infection of the upper respiratory tract in horses has previously been described as a uniformly fatal disease. As this case report illustrates, medical and surgical treatment of sinonasal cryptococcal granulomas in horses may be successful, but the importance of long-term follow-up and the potential for disease recrudescence should be considered. As efficacious antifungal agents become less expensive, their increased use will likely decrease mortality rates in horses with fungal infections.