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- Author or Editor: Dominique J. Griffon x
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Abstract
Objective—To characterize the gait of small-breed dogs walked on a pressure walkway by handlers moving at a metronome-set tempo and to determine the influence of handler and leash side on gait characteristics.
Design—Prospective study.
Animals—5 healthy adult small-breed dogs weighing < 11.4 kg (25 lb).
Procedures—Dogs were walked by each of 5 handlers moving at a metronome-set tempo (100 beats/min). Velocity, cadence, stance time, number of activated sensors, total pressure index (TPI), left or right hind reach, and symmetry indices were obtained with the leash on the left and right sides of each dog for each handler.
Results—Coefficients of variation for TPI and stance time approximated 30%, whereas coefficients of variation for symmetry indices remained < 20%. Changing handlers and leash side did not influence hind limb variables. Changing handlers influenced the TPI of the forelimbs, inducing changes of up to 8%. Leash side accounted for 12% and 14% of the variation in symmetry indices of TPI and number of sensors activated between forelimbs, respectively (mean alterations for recorded variables, 9%).
Conclusions and Clinical Relevance—Symmetry indices appeared to vary less than variables obtained for individual dog limbs, and it may therefore be advantageous to determine those indices during large trials. Handlers or leash side may be changed in studies focusing on dogs’ hind limbs without affecting results. Use of symmetry indices is recommended in forelimb studies requiring multiple handlers. Pressure walkway analyses of the forelimbs should include equal distribution of left- and right-sided leash-led trials, given that small-breed dogs tended to shift weight toward the forelimb opposite the leash.
Abstract
OBJECTIVE
To gather and evaluate veterinarians' perspectives about the impacts of the COVID-19 pandemic on the use of veterinary telehealth and on cat owners' versus dog owners' attitudes toward transmission of the SARS-CoV-2 virus from their pets.
SAMPLE
93 respondent veterinarians (47 in primary care practice and 46 in specialty practice).
PROCEDURES
An online survey was conducted between June 15 and July 15, 2020, and included 21 questions concerning demographics, use of telehealth before and after the onset of the pandemic (before March 15, 2020, and between March 15 and June 15, 2020, respectively), changes in caseloads, and perception of clients' concerns about potential for transmission of the SARS-CoV-2 virus from pets. Reported zip codes informed the collection of census data.
RESULTS
The level of poverty was significantly lower in zip code areas for respondents who reported telehealth services were (vs were not) offered before the pandemic. The percentage of respondents who reported their practice offered telehealth services increased from 12% (11/93) before the pandemic to 38% (35/93) between March 15 and June 15, 2020. Although most respondents reported owner-expressed concerns over SARS-CoV-2 virus transmission from their pets, most also reported increased caseloads, seeing newly adopted pets, and few discussions of surrender of pets for reasons related to the SARS-CoV-2 virus.
CONCLUSIONS AND CLINICAL RELEVANCE
Findings indicated that caseloads increased and telehealth services expanded during the pandemic but that there was no evidence of differences in respondent-reported owner concern for SARS-CoV-2 virus transmission from cats versus dogs.
Abstract
Objective—To evaluate effects of small intestinal submucosa (SIS) on elution properties of plaster of Paris (POP).
Sample Population—27 POP cylinders, 27 POP spheres, and 9 polymethylmethacrylate (PMMA) spheres.
Procedures—Pellets were loaded with gentamicin (50 mg/g) and divided into 7 groups of 9 beads each: PMMA spheres; POP cylinders coated with 0, 4, or 8 layers of SIS; and POP spheres coated with 0, 4, or 8 layers of SIS. Gentamicin concentration was measured 6, 12, 18, 24, 32, 40, and 48 hours and 3, 4, 5, 7, 14, 21, 28, 35, and 42 days after wrapping. Porosity was evaluated via scanning electron microscopy. Curvature factor of elution curves, total amount of drug released (TDR), time required to reach 50% of total release (TDRt50), and number of days with concentrations ≥ 1 μg/mL were compared among groups.
Results—SIS decreased the curvature factor and increased the TDRt50 and TDR of POP spheres and cylinders. Curvature factor of the PMMA-release curve remained lower than that for any POP group, but all POP groups wrapped in SIS released more gentamicin than PMMA spheres. Gentamicin concentrations remained ≥ 1 μg/mL in SIS-wrapped POP and PMMA groups throughout the study. Wrapping POP in SIS minimized the increase in porosity of pellets.
Conclusions and Clinical Relevance—Wrapping POP with SIS slows the release and increases the amount of gentamicin leaching from spheres and cylinders. All groups wrapped in SIS maintained antimicrobial concentrations greater than the minimum inhibitory concentration of most pathogens.
Abstract
Objective—To evaluate skeletal characteristics of pelvic limbs with and without cranial cruciate ligament (CCL) deficiency in Labrador Retrievers.
Animals—30 adult purebred Labrador Retrievers.
Procedures—Pelvic limbs (n = 28) of 14 dogs without CCL deficiency were classified as control limbs, whereas the limbs of 16 dogs with CCL deficiency were considered affected by (18 limbs) or predisposed to (10 contralateral limbs of dogs with 1 affected limb) CCL deficiency. Skeletal characteristics were evaluated via physical examination, radiography, and computed tomography. Radiographic and computed tomographic variables were compared among limb groups by use of a mixed-model ANOVA.
Results—The tibial plateau slope was steeper in CCL-deficient limbs but not in predisposed limbs, compared with the slope in control limbs. The angle between diaphyseal and proximal tibial axes was increased in both CCL-deficient and predisposed limbs. The relative width of the proximal portion of the tibia and the inclination of the patellar ligament did not differ among limb groups. The overall and distal femoral anteversion angles were greater in CCL-deficient and predisposed limbs, whereas the femoral condyle trochanteric angle was decreased in those limb groups, compared with findings in control limbs.
Conclusions and Clinical Relevance—Cranial angulation of the proximal portion of the tibia, excessive steepness of the tibial plateau, and distal femoral torsion appeared more likely to be associated with CCL deficiency than femoral angulation, tibial torsion, intercondylar notch stenosis, and increased inclination of the patellar ligament.
Abstract
Objective—To determine effects of sodium hyaluronate (HA) on corticosteroid-induced cartilage matrix catabolism in equine articular cartilage explants.
Sample Population—30 articular cartilage explants from fetlock joints of 5 adult horses without joint disease.
Procedure—Articular cartilage explants were treated with control medium or medium containing methylprednisolone acetate (MPA; 0.05, 0.5, or 5.0 mg/mL), HA (0.1, 1.0, or 1.5 mg/mL), or both. Proteoglycan (PG) synthesis was measured by incorporation of sulfur 35-labeled sodium sulphate into PGs, and PG degradation was measured by release of radiolabeled PGs into the medium. Total glycosaminoglycan (GAG) content in media and explants and total explant DNA were determined.
Results—Methylprednisolone acetate caused a decrease in PG synthesis, whereas HA had no effect. Only the combination of MPA at a concentration of 0.05 mg/mL and HA at a concentration of 1.0 mg/mL increased PG synthesis, compared with control explants. Methylprednisolone acetate increased degradation of newly synthesized PGs into the medium, compared with control explants, and HA alone had no effect. Hyaluronate had no effect on MPAinduced PG degradation and release into media. Neither MPA alone nor HA alone had an effect on total cartilage GAG content. Methylprednisolone acetate caused an increase in release of GAG into the medium at 48 and 72 hours after treatment. In combination, HA had no protective effect on MPA-induced GAG release into the medium. Total cartilage DNA content was not affected by treatments.
Conclusions and Clinical Relevance—Our results indicate that HA addition has little effect on corticosteroid- induced cartilage matrix PG catabolism in articular cartilage explants. (Am J Vet Res 2005;66:48–53)
Abstract
Objective—To compare 1-year outcomes after lateral fabellar suture stabilization (LFS) and tibial plateau leveling osteotomy (TPLO) for the treatment of dogs with cranial cruciate ligament disease.
Design—Randomized blinded controlled clinical trial.
Animals—80 dogs with naturally occurring unilateral cranial cruciate ligament disease.
Procedures—All dogs were randomly assigned to undergo LFS (n = 40) or TPLO (40). Clinical data collected included age, weight, body condition score, history information, stifle joint instability, radiographic findings, surgical findings, and complications. Outcome measures were determined prior to surgery and at 6 and 12 weeks and 6 and 12 months after surgery, including values of pressure platform gait analysis variables, Canine Brief Pain Inventory scores, owner satisfaction ratings, thigh circumference, and stifle joint goniometry values.
Results—Signalment and data for possible confounding variables were similar between groups. Peak vertical force of affected hind limbs at a walk and trot was 5% to 11% higher for dogs in the TPLO group versus those in the LFS group during the 12 months after surgery. Canine Brief Pain Inventory, goniometry, and thigh circumference results indicated dogs in both groups improved after surgery, but significant differences between groups were not detected. Owner satisfaction ratings at 12 months after surgery were significantly different between groups; 93% and 75% of owners of dogs in the TPLO and LFS groups indicated a satisfaction score ≥ 9 (scale, 1 to 10), respectively.
Conclusions and Clinical Relevance—Kinematic and owner satisfaction results indicated dogs that underwent TPLO had better outcomes than those that underwent LFS.
Abstract
Objective—To compare the feasibility of training veterinary medicine students to perform laparoscopic versus conventional open ovariectomy in live dogs.
Design—Randomized prospective parallel-group experiment.
Population—25 students completing the second year of their veterinary curriculum.
Procedures—Students were randomly assigned to 2 groups to receive 14 hours of specific training in either open ovariectomy (n = 13) or laparoscopic ovariectomy (12). Confidence, basic surgical skills, and basic laparoscopic skills were evaluated before and after training, prior to live surgical procedures.
Results—Scores related to basic surgical skills were high in both groups and did not improve with either training program. Before live animal surgeries, student confidence and basic laparoscopic skills improved after training in laparoscopic ovariectomy and were higher than after training in open ovariectomy. Surgery time was higher for the students who received training in laparoscopic ovariectomy (129 minutes; range, 84 to 143 minutes), compared with students who received training in open ovariectomy (80 minutes; range, 62 to 117 minutes). On a 55-point scoring system, ovariectomy scores were similar between students who received training in open ovariectomy (34.5; range, 16.5 to 45) and students who received training in laparoscopic ovariectomy (34.5; range, 25 to 44.5).
Conclusions and Clinical Relevance—The training programs were effective in improving student confidence and skills in laparoscopic ovariectomy. Results of this study suggested that veterinary medical students, with assistance from an instructor, may be taught to perform laparoscopic ovariectomies with performance equivalent to that for students performing open ovariectomies.
Abstract
Objectives—To compare combined vacuum and rotation with the spinner flask technique for seeding chondrocytes on chitosan versus polyglycolic acid matrices.
Sample Population—Porcine chondrocytes.
Procedure—A suspension containing 5 × 106 chondrocytes/ scaffold was used to evaluate 2 seeding techniques, including a spinner flask and a customdesigned vacuum chamber used for 2 hours prior to transfer to a bioreactor. For each seeding technique, prewetted scaffolds were composed of polyglycolic acid (PGA) mesh or macroporous chitosan sponge. Constructs were collected at 48 hours for DNA quantification, measurement of water and gycosaminoglycan (GAG) content, and scanning electron microscopy.
Results—Yield of both seeding techniques was similar for each type of scaffold. Percentage of cells contained in the center of PGA constructs was increased with seeding in the bioreactor (43% of total cell number), compared with the spinner flask (18%). The DNA content and cell number per construct were 10 times greater for PGA constructs, compared with chitosan constructs. Chitosan scaffolds seeded in the bioreactor yielded a significantly higher GAG:DNA ratio than did PGA scaffolds. Whereas chondrones formed on chitosan scaffolds, cell distribution was more uniform on PGA scaffolds.
Conclusions and Clinical Relevance—The vacuumbioreactor technique allowed seeded chondrocytes to attach to PGA scaffolds within 48 hours and improved uniformity of cell distribution, compared with the spinner technique. Although formation of extracellular matrix may be stimulated by seeding chitosan scaffolds in the bioreactor, further evaluations of the seeding technique and characteristics of chitosan scaffolds are warranted. (Am J Vet Res 2005;66:599–605)
Abstract
Objective—To establish the dose-dependent effects of high-molecular-weight hyaluronic acid (HA) supplementation on chondrogenesis by mesenchymal stem cells (MSCs) cultured on chitosan sponges and to determine the extent to which MSC matrix production (chondrogenesis) can be influenced by incorporation of high-molecular-weight HA into chitosan scaffolds.
Sample Population—Murine MSCs derived from a multipotent bone marrow stromal precursor.
Procedures—MSCs were seeded on chitosan and chitosan-HA scaffolds in chondrogenic medium with various HA concentrations. Scanning electron microscopy, fluorescence microscopy (viability assay), and DNA quantification were used to assess cell attachment, distribution, and viability 48 hours after seeding. Constructs were cultured for 3 weeks prior to evaluation of cell distribution and chondrogenic differentiation via histologic evaluation and quantification of DNA, glycosaminoglycan, and collagen II.
Results—48 hours after MSC seeding, cell viability and DNA content were similar among groups. Three weeks after seeding, HA supplementation of the culture medium improved matrix production in a dose-dependent manner, as indicated by matrix glycosaminoglycan and collagen II concentrations. The scaffold composition, however, had no significant effect on matrix production.
Conclusions and Clinical Relevance—High-molecular-weight HA supplementation in culture medium had a dose-dependent effect on matrix production and thus chondrogenic differentiation of MSCs cultured on chitosan sponges. The addition of HA in the surrounding fluid during chondrogenesis should improve cartilage production and may be useful for producing engineered cartilage tissues.
