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  • Author or Editor: Desireè L. Lipscomb x
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Abstract

Objective—To determine the nucleotide sequence of the α IIb gene from canine platelet-derived cDNA.

Animals—3 adult dogs.

Procedure—First-strand cDNA was prepared from total RNA isolated from canine platelets. The cDNA was amplified, using specific primers in polymerase chain reaction (PCR), and the nucleotide sequence was obtained from purified PCR products.

Results—Except for the nucleotide at position 694, results of all sequencing reactions of α IIb were identical for canine platelet-derived cDNA. Canine α IIb had 3 fewer codons than α IIb of humans. The nucleotide and deduced amino acid sequences of full-length canine α IIb shared ≥ 83% similarity with the sequences established for humans. Segments of canine α IIb nucleotide and deduced amino acid sequences were ≥ 78% similar to α IIb associated with 7 functional domains (extracellular, transmembrane, cytoplasmic, and 4 calcium-binding domains) in humans, with the highest degree of similarity correlating with the sequences of the 4 calcium-binding domains. Amino acid residues associated with development of alloantibodies in humans (Met837, Val837, Ile843, Ser843) are not encoded by canine α IIb .

Conclusions and Clinical Relevance—The nucleotide variation at position 694 of canine α IIb may represent a polymorphism. The species differences in the α IIb sequence may contribute to variations in receptor-li gand interactions. The high degree of α IIb sequence conservation of the 4 calcium-binding domains implies functional importance. Some disorders associated with α IIb β3 in dogs are clinically analogous to diseases in humans, and results indicate that dogs are an appropriate model for the evaluation of gene therapy and other treatments of platelet-associated disorders. (Am J Vet Res 2001;62:1486–1492)

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in American Journal of Veterinary Research