Objective—To assess the outcome of extrahepatic portosystemic shunt (EHPSS) treatment in dogs aged 5 years and older.
Design—Retrospective case series.
Animals—17 client-owned dogs.
Procedures—Medical records for dogs (≥ 5 years old) that underwent surgical attenuation of an EHPSS (1992 through 2005) were evaluated; data, including clinical signs, clinicopathologic findings, surgical procedure, and outcome, were recorded. Follow-up information was obtained via patient examination or telephone interview with veterinarians and owners.
Results—Dogs (5 to 9 years old [median age, 6.6 years]) had neurologic (n = 12), urinary tract (8), and gastrointestinal tract (6) EHPSS-associated clinical signs. Serum bile acids and ammonia concentrations were abnormal in all evaluated dogs. Treatment of EHPSSs included complete (n = 6 dogs) or partial (2) suture attenuation or ameroid constrictor placement (9). Two dogs died following surgery. Follow-up information (6 to 120 months) was available for 13 dogs. Deaths were attributable to heart failure (n = 1), bacterial hepatitis (2; with pyelonephritis in 1 dog), and unknown causes (3). At a median of 23 and 25 months, serum bile acids concentrations had almost normalized in 5 of 8 dogs and ammonia concentrations were within reference limits in 3 of 5 dogs, respectively; dogs with abnormal liver function test results had no associated clinical signs. Median long-term survival time was 72 months.
Conclusions and Clinical Relevance—Attenuation of EHPSS in ≥ 5-year-old dogs ameliorated signs of liver dysfunction in surviving dogs, although return of normal liver function occurred less frequently than expected.
Objective—To describe the use of thoracoscopic-assisted pulmonary surgery (TAPS) for partial and complete lung lobectomy in small animal patients and to evaluate short-term outcome.
Design—Retrospective case series.
Animals—11 client-owned dogs and cats.
Procedures—Medical records of dogs and cats that underwent a partial or complete TAPS lung lobectomy were reviewed. All patients underwent general anesthesia and were positioned in lateral recumbency with the affected hemithorax uppermost. One-lung ventilation was not implemented in any patient. For initial exploration, a 5- to 10-mm incision was made for insertion of a 30° telescope approximately 5 to 7 rib spaces away from the site of the pulmonary lesion in the dorsal third of the thorax. All subsequent incision placements were case dependent and determined by the location of the lesion to be resected. Following lesion localization, a 2- to 7-cm minithoracotomy incision was made with direct thoracoscopic visualization without the use of rigid rib retractors. In 10 of 11 patients, a 360° wound retraction device was placed at the minithoracotomy site prior to exteriorization and resection of the affected lung. Lymph nodes were inspected intraoperatively, but biopsies were not performed; incisions were closed routinely, and a thoracostomy tube was placed in all patients.
Results—3 cats and 8 dogs underwent successful partial (5) or complete (6) TAPS lung lobectomy over a 5-year period (2008 through 2013). Median surgery time was 92.7 minutes (range, 77 to 150 minutes). Thoracostomy tubes were removed a median of 22.3 hours after surgery (range, 18 to 36 hours). The median time to discharge was 3.1 days (range, 1 to 6 days). No intraoperative complications were encountered. All patients were discharged from the hospital, with 9 of 11 patients alive 6 months after surgery.
Conclusions and Clinical Relevance—Results of this study suggested that lung lobectomy by means of TAPS can be successfully performed in dogs and cats. When compared with total thoracoscopic surgery, TAPS may offer a more technically feasible approach from both a surgical and anesthetic standpoint, because it provides the benefits of minimally invasive thoracic surgery without the necessity of 1-lung ventilation.
Objectives—To determine the role of myosin light
chain phosphorylation in feline colonic smooth muscle
Sample Population—Colonic tissue was obtained
from eight 12- to 24-month-old cats.
Procedure—Colonic longitudinal smooth muscle
strips were attached to isometric force transducers
for measurements of isometric stress. Myosin light
chain phosphorylation was determined by isoelectric
focusing and sodium dodecyl sulfate-polyacrylamide
gel electrophoresis. Stress and phosphorylation were
determined following stimulation with ACh or SP, in
the absence or presence of a calmodulin antagonist
(W-7; 0.1 to 1.0 mM), myosin light chain kinase
inhibitor (ML-9; 1 to 10 µM), or extracellular calcium -
Results—Unstimulated longitudinal colonic smooth
muscle contained low amounts (6.9 ± 3.2%) of phosphorylated
myosin light chain. Phosphorylation of the
myosin light chains was dose and time dependent
with maximal values of 58.5% at 30 seconds of stimulation
with 100 µM Ach and 60.2% at 45 seconds of
stimulation with 100 nM SP. Active isometric stress
development closely paralleled phosphorylation of the
myosin light chains in ACh- or SP-stimulated muscle.
W-7 and ML-9 dose dependently inhibited myosin light
chain phosphorylation and isometric stress development
associated with ACh or SP stimulation. Removal
of extracellular calcium inhibited myosin light chain
phosphorylation and isometric stress development in
ACh-stimulated smooth muscle.
Conclusions and Clinical Relevance—Feline longitudinal
colonic smooth muscle contraction is
calcium-, calmodulin-, and myosin light chain kinasedependent.
Myosin light chain phosphorylation is necessary
for the initiation of contraction in feline longitudinal
colonic smooth muscle. These findings may
prove useful in determining the biochemical and molecular
defects that accompany feline colonic motility
disorders. (Am J Vet Res 2002;63:695–702)
Objective—To determine whether expression of G
proteins (Gi and Gs) is altered in thyroid gland adenomas
obtained from hyperthyroid cats.
Sample Population—Adenomatous thyroid glands
obtained from 8 hyperthyroid cats and thyroid glands
obtained from 4 age-matched euthyroid cats.
Procedure—Expression of Gi and Gs was quantified
in enriched membrane preparations of thyroid gland
tissue, using immunoblotting with Gi and Gs antibodies
and toxin-catalyzed ADP-ribosylation.
Results—Expression of Gi was significantly reduced
in thyroid gland adenomas from hyperthyroid cats,
compared with normal thyroid gland tissue from
euthyroid cats. Expression of Gs was similar between
the 2 groups.
Conclusions and Clinical Relevance—A decrease in
expression of Gi in adenomatous thyroid glands of
cats may reduce the negative inhibition of the cAMP
cascade in thyroid cells, leading to autonomous
growth and hypersecretion of thyroxine.
Understanding the molecular mechanisms for hyperthyroidism
in cats may lead to better treatment or,
ultimately, prevention of the disease. (Am J Vet Res
To evaluate complication rates for various types of mastectomy procedures, identify factors associated with an increased risk of complications, and determine the consequences of such complications.
140 female dogs that underwent 154 separate mastectomy procedures to treat mammary gland tumors.
Medical records of dogs in the Penn Vet Shelter Canine Mammary Tumor Program from July 2009 to March 2015 were reviewed. Data regarding signalment, tumor characteristics (ie, number and size, benign or malignant, and bilateral or unilateral), mastectomy type, anesthesia time, concurrent ovariohysterectomy or ovariectomy, surgeons’ qualifications, antimicrobial administration after surgery, postoperative placement of surgical drains, and complications (seroma, abscess, dehiscence, or infection) were collected. Complications that required hospitalization were recorded. Fisher exact tests were used to evaluate associations between variables of interest and complications. Multivariable analysis was used to identify factors independently associated with an increased risk of complications.
Complication rate following all mastectomy procedures was 16.9% (26/154); of these, 9 (34.6%) required hospitalization. High body weight, undergoing bilateral mastectomy, and postoperative antimicrobial administration were associated with significantly increased odds of complications. The odds of complications associated with postoperative antimicrobial administration, however, varied according to mastectomy type; dogs undergoing chain mastectomy that did not receive antimicrobials postoperatively had the highest odds of developing complications. Dogs undergoing concurrent ovariohysterectomy or ovariectomy had significantly decreased odds of complications.
CONCLUSIONS AND CLINICAL RELEVANCE
Previously spayed dogs with a large body size that underwent the most extensive mastectomy procedures had increased odds of having postoperative complications.
Objective—To determine the prevalence of nonneoplastic middle ear disease among cats undergoing necropsy and the prevalence of clinical abnormalities in cats in which nonneoplastic middle ear disease was identified.
Design—Retrospective case series.
Animals—59 cats that underwent necropsy between January 1991 and August 2007.
Procedures—Medical records were searched to identify cats in which nonneoplastic middle ear disease was identified at necropsy. For cats included in the study, data that were recorded included signalment, initial complaint, whether the cat had any clinical signs of middle or external ear disease, whether the cat had upper respiratory tract disease, necropsy diagnosis, gross appearance of the bullae, and reason for euthanasia. Signs of middle ear disease that were considered included unilateral peripheral vestibular disease without motor deficits, Horner syndrome, and facial nerve paralysis.
Results—Of the 3,442 cats that underwent necropsy during the study period, 59 (1.7%) had nonneoplastic middle ear disease. Six of the 59 (10%) cats, including 1 cat that was affected bilaterally, had clinical signs of middle ear disease. Of these, 5 had signs of unilateral peripheral vestibular disease, and 1 had Horner syndrome.
Conclusions and Clinical Relevance—Results suggested that most cats with nonneoplastic middle ear disease did not have associated clinical signs. Findings may be of clinical relevance for cats in which middle ear disease is identified as an incidental finding during computed tomography or magnetic resonance imaging for unrelated diseases.
Objective—To determine the prevalence of exposure to canine influenza virus (CIV) in dogs in a metropolitan animal shelter.
Procedures—Dogs were randomly selected from the canine shelter population. A physical examination was performed, and blood samples were obtained and submitted for serologic testing for the detection of antibodies against CIV. Logistic regression analysis was performed to evaluate the association of factors (body condition score, nasal discharge, coughing, rectal temperature, number of days in the shelter, and relinquished vs stray) with positive results.
Results—31 of 74 (42%) dogs were seropositive for antibodies against CIV. Positive serologic test results were detected for 6 of 39 (15%) dogs housed in the shelter for ≤ 7 days and for 25 of 35 (71%) dogs housed in the shelter for ≥ 8 days. Number of days in the shelter was the only factor significantly associated with positive serologic test results. For every 3 days in the shelter, the odds of a positive serologic test result increased significantly by 2.2 (95% confidence interval, 1.5 to 3.4).
Conclusions and Clinical Relevance—Analysis of the results suggested that more dogs were exposed to CIV in the shelter than were exposed in the urban environment. This has serious implications for design and management of animal shelters.
OBJECTIVE To sequence exons and splice consensus sites of the dynactin subunit 1 (DCTN1) gene in Leonbergers and Labrador Retrievers with clinical laryngeal paralysis.
ANIMALS 5 unrelated Leonbergers with laryngeal paralysis, 2 clinically normal Leonbergers, 7 unrelated Labrador Retrievers with laryngeal paralysis, and 2 clinically normal Labrador Retrievers.
PROCEDURES Primers were designed for the entire coding regions of the DCTN1 gene, a noncoding exon at the 5´ end of the gene, and a 900-bp single-nucleotide polymorphism (SNP)-rich region located 17 kb upstream of the DCTN1 gene by use of the CanFam3 assembly of the canine genome sequence. Sequences were generated and compared between clinically normal and affected dogs. The SNPs flanking the DCTN1 gene as well as a previously identified nonsynonymous SNP in exon 32 were genotyped in affected and clinically normal Leonbergers and Labrador Retrievers.
RESULTS None of the affected dogs were homozygous for any mutation affecting coding regions or splicing consensus sequences. Of the 16 dogs tested for the missense SNP in exon 32, all were homozygous for the reference allele, except for 2 affected and 1 clinically normal Labrador Retriever and 1 clinically normal Leonberger. The DCTN1 gene sequences (5 dogs) and haplotypes of polymorphic markers surrounding the DCTN1 gene (all dogs) were not consistent with the hypothesis that laryngeal paralysis was associated with inheritance of the same DCTN1 disease-causing allele within all Labrador Retrievers or Leonbergers evaluated.
CONCLUSIONS AND CLINICAL RELEVANCE Mutations in the DCTN1 gene did not appear to cause laryngeal paralysis in Leonbergers or Labrador Retrievers.
Objective—To identify within guanosine triphosphate–binding proteins (G proteins) the subset of
inhibitory G proteins (Gi) that have decreased expression
in adenomatous thyroid glands obtained from
Sample Population—Adenomatous thyroid glands
obtained from 5 hyperthyroid cats and normal thyroid
glands obtained from 3 age-matched euthyroid cats.
Procedure—Expression of Gi1, Gi2, and Gi3 in
enriched membrane preparations from thyroid glands
was quantified by use of immunoblotting with Gi subtype-specific antibodies.
Results—Expression of Gi2 was significantly
decreased in tissues of hyperthyroid glands, compared
with expression in normal thyroid tissue.
Expression of Gi1 and Gi3 was not significantly different
between normal thyroid tissues and tissues from
Conclusions and Clinical Relevance—A decrease in
Gi2 expression decreases inhibition of adenylyl
cyclase and allows a relative increase in stimulatory G
protein expression. This results in increased amounts
of cAMP and subsequent unregulated mitogenesis
and hormone production in hyperthyroid cells.
Decreased Gi2 expression may explain excessive
growth and function of the thyroid gland in cats with
hyperthyroidism. (Am J Vet Res 2005;66:1478–1482)
Objective—To describe a method for ultrasonographic and color-flow Doppler ultrasonographic imaging of the 4 direct cutaneous arteries commonly used for axial pattern skin flaps in dogs.
Animals—20 clinically normal dogs.
Procedures—Dogs were manually restrained and fundamental and harmonic ultrasonographic and colorflow Doppler ultrasonographic examinations of the superficial cervical, thoracodorsal, deep circumflex iliac (cranial and caudal branches), and caudal superficial epigastric arteries were performed by a resident in diagnostic imaging. The level of confidence in locating these vessels was subjectively graded as high, moderate, or low.
Results—High-frequency fundamental and harmonic ultrasonography was important for maximizing image resolution, and color-flow Doppler ultrasonography was important for vessel identification. The superficial cervical artery was the most difficult vessel to identify; confidence in correct vessel identification was low or moderate. The thoracodorsal and deep circumflex iliac arteries were identified with a moderate or high level of confidence. The caudal superficial epigastric artery was the easiest vessel to identify; confidence in correct vessel identification was high. Except for the superficial cervical artery, the level of confidence in correct vessel identification improved over time as operator experience increased.
Conclusion and Clinical Relevance—Results suggest that the combination of fundamental ultrasonographic and color-flow Doppler ultrasonographic imaging is an easy and noninvasive method for identifying the 4 direct cutaneous arteries commonly used for axial pattern skin flaps in dogs. This method could be useful in planning axial pattern skin flaps, particularly in dogs with regional soft tissue trauma in which the integrity of the vessel is in question.