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in Journal of the American Veterinary Medical Association


Virulence factors were studied in 82 strains of Escherichia coli isolated from the urine of dogs with urinary tract infections. The most frequently expressed O antigens were 2, 4, 6, 25, and 22/83. Most strains were K nontypeable. Mannose-sensitive hemagglutination (msh) with canine erythrocytes was observed in 71 strains and mannose-resistant hemagglutination (mrh) was observed in 32 strains. Strains that caused msh of erythrocytes from dogs also caused msh of erythrocytes from guinea pigs. Most strains that caused mrh of human A1p1 erythrocytes also reacted with erythrocytes of dogs.

Of 22 strains (27%)_that agglutinated human A1P1 erythrocytes, but not A1P1 erythrocytes, 17 (77%) had specificity for globo A, but did not react with the galactose α1→4galactoseβ disaccharide receptor. The remaining 5 strains and 2 others that simultaneously expressed an X adhesin agglutinated galactose α1→4galactoseβ-coated latex beads.

Bacterial adherence to canine uroepithelial cells from the bladder was most often observed in strains expressing msh, less often observed in strains expressing mrh, and least often observed in strains that failed to induce hemagglutination. Adherence of msh strains to canine uroepithelial cells was inhibited by α-methyl-D-mannoside. As a group, mrh strains expressing globo-A-and galactose α1→4galactoseβ-specific adhesins did not have strong adherence.

Strains of E coli isolated from dogs with urinaiy tract infections most commonly expressed type-1 fimbriae, and the main mechanism of in vitro adherence to canine uroepithelial cells involved a mannose-sensitive mechanism. Overrepresentation of globo-A-specific adhesins did not appear to be related to adherence of canine uroepithelial cells.

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in American Journal of Veterinary Research