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Summary

Bone marrow blood and venous blood were collected from 21 healthy cats for analysis of 13 serum biochemical variables. The relationship between values in venous serum and bone marrow serum for each variable was evaluated, using a linear regression F test. A P value ≤ 0.05 was considered significant evidence that measurements in venous and marrow serum were correlated for that biochemical variable. Biochemical variables for which values were significantly correlated included urea nitrogen, creatinine, cholesterol, total protein, albumin, sodium, chloride, and phosphorus concentrations. In all cats, activities for alanine transaminase and alkaline phosphatase in bone marrow serum exceeded the activities in venous serum. Potassium and phosphorus concentrations were greater in bone marrow serum than in venous serum in 20 cats. Glucose concentrations in bone marrow serum from 19 cats were lower than those in venous serum. Correlation between calcium concentrations in venous and bone marrow serum was not found.

Free access
in Journal of the American Veterinary Medical Association
in Journal of the American Veterinary Medical Association
in Journal of the American Veterinary Medical Association

Abstract

Objective

To determine whether plasma von Willebrand factor (vWf) concentration changes in horses during and after treadmill exercise.

Animals

5 mature, fit Thoroughbreds.

Procedure

A blood sampling catheter was placed in the right jugular vein. A warm-up period was followed by a 3-minute rest period. Horses were galloped at racing pace until fatigued (about 2 minutes). Blood samples were collected prior to warm-up, during the postwarm-up rest period, 1 minute into the run, at cessation of the run, and 5 to 120 minutes after cessation of the run. vWF activity was measured by ELISA and corrected for plasma volume changes (measured by changes in plasma albumin concentration). Platelet-poor plasma from 10 clinically normal, resting horses was pooled, assigned a value of 100 U/dl, and served as a control for all assays.

Results

vWf activity began increasing 1 minute after horses reached full speed. At 5 minutes after cessation of exercise, vWf values had increased by mean of 92% (P < 0.05) from baseline. vWf activity returned to baseline by 15 minutes after exercise, and remained there until 90 minutes after exercise, when it began to increase.

Conclusion and Clinical Relevance

The spontaneous decrease in vWf values after completion of exercise was unexpected because vWf has a long half-life in circulation. This unexpected finding is compatible with increased vWf consumption and suggests that microvascular trauma may occur in horses during strenuous exercise. (Am J Vet Res 1997;58:71–76)

Free access
in American Journal of Veterinary Research