Objective—To determine diagnostic accuracy of a compartmented bacteriologic culture and antimicrobial susceptibility testing plate (CCSP) for detection of bacterial urinary tract infection (UTI) in dogs and antimicrobial susceptibility testing of bacterial isolates.
Sample—62 frozen, previously characterized bacterial isolates from canine urine cultures and 147 canine urine samples.
Procedures—The study was conducted in 2 phases: preliminary assay validation (phase 1) and diagnostic validation (phase 2). For phase 1, the frozen bacterial isolates were revitalized and tested with the CCSP and with standard aerobic microbiological culture (SAMC). For phase 2, the urine samples were tested with the CCSP and SAMC in parallel.
Results—For phase 1, after 24 hours of culture, 46 of 62 (74%) bacterial isolates had growth on the CCSP and all (100%) had growth in SAMC. For bacterial isolates with growth, the CCSP allowed correct identification of 45 of 46 (98%) isolates. Isolates yielding no growth on the CCSP were gram-positive cocci (Staphylococcus spp [n = 7] and Enterococcus spp ). In phase 2, the overall diagnostic accuracy of the CCSP, compared with SAMC, was 94% (sensitivity, 81%; specificity, 99%). The positive predictive value was 98% and negative predictive value was 92%. Susceptibility results for enrofloxacin and trimethoprim-sulfamethoxazole as determined with the CCSP had greatest concordance with those determined by SAMC (71% and 96%, respectively), compared with other antimicrobial susceptibilities.
Conclusions and Clinical Relevance—Use of the CCSP led to accurate exclusion of UTI in dogs without a UTI but was less reliable for diagnosis of UTI, particularly infections caused by gram-positive cocci. Standard aerobic microbiological culture remains the gold standard for detection of UTI in dogs.
Objective—To determine frequency with which
Staphylococcus schleiferi could be isolated from dogs
with pyoderma and antimicrobial susceptibility patterns
of isolates that were obtained.
Animals—54 dogs with a first (n = 14) or recurrent
(40) episode of pyoderma.
Procedure—Specimens were obtained and submitted
for bacterial culture. Isolates were identified as S
schleiferi on the basis of growth and biochemical
characteristics. Two isolates were submitted for DNA
sequencing to confirm identification. Methicillin susceptibility
was determined by means of disk diffusion
with oxacillin-impregnated disks.
Results—3 of 14 dogs examined because of a first
episode of pyoderma and 12 of 40 dogs examined
because of a recurrent episode of pyoderma were
receiving antimicrobials at the time of specimen collection.
Staphylococcus schleiferi was not isolated
from any dog with first-time pyoderma but was isolated
from 5 dogs with recurrent pyoderma that were
not receiving antimicrobials at the time of specimen
collection and 10 dogs with recurrent pyoderma that
were receiving antimicrobials. Nine isolates were
identified as S schleiferi subsp schleiferi, and 6 were
identified as S schleiferi subsp coagulans. All S
schleiferi subsp schleiferi isolates were resistant to
methicillin, but only 2 S schleiferi subsp coagulans
isolates were. Two methicillin-resistant isolates were
also resistant to fluoroquinolones, and 1 isolate had
intermediate susceptibility to fluoroquinolones.
Conclusions and Clinical Relevance—Results suggest
that S schleiferi subsp schleiferi and S schleiferi
subsp coagulans may be isolated from dogs with recurrent
pyoderma. Although isolates from dogs with pyoderma
were frequently resistant to methicillin, multiple
drug resistance was uncommon. (J Am Vet Med Assoc
Objective—To determine whether resistance to oxacillin and other antimicrobials in 3 Staphylococcus spp commonly isolated from dogs increased from 2001 to 2005.
Design—Retrospective case series.
Sample Population—1,772 clinical samples of various types obtained from dogs examined at the University of Tennessee Veterinary Teaching Hospital or at regional veterinary hospitals and submitted to the bacteriology and mycology laboratories associated with the teaching hospital.
Procedures—Samples were submitted by attending veterinarians to the bacteriology and mycology laboratories for routine aerobic microbial culture. Identification and antimicrobial susceptibility procedures were performed on all isolates. Susceptibility reports for each antimicrobial and Staphylococcus spp were determined from aggregate electronically archived test results. Oxacillin and multidrug resistance for Staphylococcus intermedius was analyzed by reviewing disk diffusion zone measurements.
Results—Oxacillin resistance increased among S intermedius isolates during the past 5 years, and the increase was associated with multidrug resistance. In 2005, 1 in 5 Staphylococcus spp isolates from canine clinical samples was resistant to oxacillin. The most common staphylococcal species isolated were S intermedius (n = 37), Staphylococcus schleiferi (21), and Staphylococcus aureus (4), and frequencies of oxacillin resistance in isolates of these species were 15.6%, 46.6%, and 23.5%, respectively.
Conclusions and Clinical Relevance—Veterinarians should be aware of the potential for empiric drug treatment failures in instances where Staphylococcus spp infections are common (eg, pyoderma). Judicious use of bacterial culture and susceptibility testing is recommended.
Objective—To determine the methicillin-resistant
profile of staphylococcal isolates from the skin of
dogs with pyoderma.
Animals—90 dogs with pyoderma.
Procedure—Staphylococci isolated from dogs with
pyoderma were tested for susceptibility to methicillin
by use of a standard disk diffusion test with oxacillin
disks. The DNA extracted from the isolates was tested
for the mecA gene that encodes the penicillinbinding
protein 2a (PBP2a) by use of a polymerase
chain reaction (PCR) assay. The expression of PBP2a
was determined with a commercial latex agglutination
assay. Species of staphylococcal isolates were
identified by use of morphologic, biochemical, and
Results—Most of the isolated staphylococci were
Staphylococcus intermedius isolates. Whereas only 2
of 57 S intermedius isolates were resistant to methicillin,
approximately half of the isolates had the mecA
gene and produced PBP2a. Staphylococcus schleiferi
was the second most common isolate. Widespread
resistance to methicillin was found among S schleiferi
isolates. More coagulase-negative S schleiferi isolates
were identified with mecA gene-mediated resistance
to methicillin, compared with coagulase-positive S
Conclusions and Clinical Relevance—The latex
agglutination assay for the detection of PBP2a
expression coupled with the PCR assay for the mecA
gene may provide new information about emerging
antimicrobial resistance among staphylococcal isolates.
(Am J Vet Res2004;65:1265–1268)
Objective—To determine the frequency of isolation and
susceptibility patterns of Staphylococcus schleiferi from
healthy dogs and dogs with otitis, pyoderma, or both
that had or had not received antimicrobial treatment.
Procedure—Dogs were allocated to 1 of 4 groups:
healthy dogs (n = 13), dogs without otitis but with pyoderma
(10), dogs with otitis but without pyoderma (11),
and dogs with otitis and pyoderma (16). Bacteriologic
culture of ear swab specimens was performed in all
dogs. Bacteriologic culture of skin swab specimens
was also performed in dogs with concurrent pyoderma.
Isolates were identified as S schleiferi subsp schleiferi
or S schleiferi subsp coagulanson the basis of growth
and biochemical characteristics.
Results—S schleiferi was not isolated from any dogs with
pyoderma only. Staphylococcus schleiferi subsp schleiferi
was isolated from the ears of 2 healthy dogs, and the skin
and ears of 2 dogs and the skin of 1 dog with otitis and
pyoderma. Staphylococcus schleiferi subsp coagulans
was isolated from the ears of 3 dogs with otitis only, and
the ears of 6 dogs and the skin of 2 dogs with otitis and
pyoderma. One of the S schleiferi subsp schleiferi isolates
from ears, 2 of the S schleiferi subsp coagulansisolates
from ears, and 1 of the S schleiferi subsp coagulansisolates
from the skin were resistant to methicillin. One
methicillin-resistant isolate from the ears and 1 from the
skin were also resistant to fluoroquinolones.
Conclusions and Clinical Relevance—S schleiferi
subsp schleiferiwas detected in healthy dogs and dogs
with otitis and pyoderma. Methicillin-resistant and -susceptible
S schleiferi subsp schleiferi and S schleiferi
subsp coagulans were detected as the predominant
organisms in dogs with otitis. ( J Am Vet Med Assoc 2005;227:928–931)
Objective—To determine prevalence of within-household sharing of fecal Escherichia coli between dogs and their owners on the basis of pulsed-field gel electrophoresis (PFGE), compare antimicrobial susceptibility between isolates from dogs and their owners, and evaluate epidemiologic features of cross-species sharing by use of a questionnaire.
Sample Population—61 healthy dog-owner pairs and 30 healthy control humans.
Procedures—3 fecal E coli colonies were isolated from each participant; PFGE profiles were used to establish relatedness among bacterial isolates. Susceptibility to 17 antimicrobials was determined via disk diffusion. A questionnaire was used to evaluate signalment, previous antimicrobial therapy, hygiene, and relationship with dog.
Results—A wide array of PFGE profiles was observed in E coli isolates from all participants. Within-household sharing occurred with 9.8% prevalence, and across-household sharing occurred with 0.3% prevalence. No behaviors were associated with increased clonal sharing between dog and owner. No differences were found in susceptibility results between dog-owner pairs. Control isolates were more likely than canine isolates to be resistant to ampicillin and trimethoprim-sulfamethoxazole. Owners and control humans carried more multdrug-resistant E coli than did dogs.
Conclusions and Clinical Relevance—Within-household sharing of E coli was detected more commonly than across-household sharing, but both direct contact and environmental reservoirs may be routes of cross-species sharing of bacteria and genes for resistance. Cross-species bacterial sharing is a potential public health concern, and good hygiene is recommended.
Objective—To determine the prevalence of 4 urovirulence genes in fecal Escherichia coli isolates from healthy dogs and their owners and to determine whether detection of E coli strains with these genes was associated with a history of urinary tract infection (UTI).
Sample Population—61 healthy dog-owner pairs and 30 healthy non–dog owners.
Procedures—A fecal specimen was obtained from each participant, and 3 colonies of E coli were isolated from each specimen. A multiplex PCR assay was used to detect 4 genes encoding virulence factors: cytotoxic necrotizing factor (cnf), hemolysin (hlyD), s-fimbrial and F1C fimbriae adhesin (sfa/foc), and pilus associated with pyelonephritis G allele III (papGIII). Human participants completed a questionnaire to provide general information and any history of UTI for themselves and, when applicable, their dog.
Results—26% (16/61) of dogs, 18% (11/61) of owners, and 20% (6/30) of non–dog owners had positive test results for ≥ 1 E coli virulence gene. One or more genes were identified in fecal E coli isolates of both dog and owner in 2% (1/61) of households. There was no difference in the detection of any virulence factor between dog-owner pairs. Female owner history of UTI was associated with detection of each virulence factor in E coli strains isolated from their dogs' feces.
Conclusions and Clinical Relevance—Dogs and humans harbored fecal E coli strains possessing the genes cnf, hlyD, sfa/foc, and papGIII that encode urovirulence factors. It was rare for both dog and owner to have fecal E coli strains with these virulence genes.
Objective—To evaluate knowledge, attitudes, and management practices involving antimicrobial use among Tennessee beef producers.
Sample Population—A population-based, stratified random sample of 3,000 beef producers across the state.
Procedures—Questionnaires were mailed to beef producers. Questions focused on producer practices related to education, biosecurity, veterinary use, and the purchase and use of antimicrobials. Operation types were categorized as either cow-calf only or multiple operation type (MOT). Associations between various factors and antimicrobial use were evaluated by use of multivariable logistic regression, with the outcome variable being any antimicrobial use (injectable or by mouth) in the past year.
Results—Of 3,000 questionnaires mailed, 1,042 (34.7%) were returned. A significantly higher proportion of producers with MOTs reported giving antimicrobials by mouth or by injection than did producers with cow-calf only operations. In addition, higher proportions of producers with MOTs than producers with cow-calf only operations reported treating with macrolides, florfenicol, ceftiofur, and aminoglycosides. In the multivariable analysis, herd size >50 cattle, participation in Beef Quality Assurance or master beef producer certification programs, quarantining of newly purchased animals, use of written instructions for treating disease, and observation of withdrawal times were associated with a higher likelihood of antimicrobial use.
Conclusions and Clinical Relevance—Results suggested that producers who engaged in more progressive farming practices were also more likely to use antimicrobials. Incorporating training on judicious antimicrobial use into educational programs would likely increase awareness of best management practices regarding antimicrobial use.