Objective—To evaluate the effects of various storage
conditions on one-stage prothrombin time (OSPT),
activated partial thromboplastin time (APTT), and fibrinogen
concentration of canine plasma collected for
Sample Population—Plasma from 9 dogs.
Procedure—Whole blood was collected from dogs by
means of jugular venipuncture and centrifuged at
7,300 × g for 20 minutes at 0 C. A plasma extractor
was then used to generate plasma. Aliquots of plasma
were collected in segments of plastic tubing and
in microcentrifuge tubes, and plasma collection bags,
tubing segments, and microcentrifuge tubes were
immediately frozen at –30 C. Additional tubing segments
and microcentrifuge tubes were stored at 2 C.
After 1 week of storage, all samples were thawed,
and OSPT, APTT, and fibrinogen concentration were
measured. Collection bags and microcentrifuge tubes
were refrozen at –30 C, and values were measured
again 30 days after blood collection.
Results—Values for OSPT, APTT, and fibrinogen concentration
did not vary significantly with storage time,
temperature, or container.
Conclusions and Clinical Relevance—Results suggested
that storage for up to 30 days and at 2 C versus
–30 C did not have any significant effect on hemostatic
parameters of canine plasma obtained for transfusion.
(Am J Vet Res 2001;62:734–735)
Objective—To compare WBC, neutrophil, and platelet counts and Hct values obtained with a point-of-care hematology analyzer with values obtained by a reference method for dogs and cats receiving chemotherapy.
Animals—105 dogs and 25 cats undergoing chemotherapy.
Procedures—Blood samples were analyzed with a point-of-care hematology analyzer and with an impedance- and laser-based analyzer with manual differential WBC counts. Results for WBC, neutrophil, and platelet counts and Hct were compared. Sensitivity and specificity of the point-of-care analyzer to detect leukopenia, neutropenia, and anemia were calculated.
Results—554 canine and 96 feline blood samples were evaluated. Correlation coefficients for dogs and cats, respectively, were 0.92 and 0.95 for total WBC count, 0.91 and 0.88 for neutrophil count, 0.95 and 0.92 for Hct, and 0.93 and 0.71 for platelet count. Sensitivity and specificity, respectively, of the point-of-care analyzer to detect leukopenia were 100% and 75% for dogs and 100% and 68% for cats; to detect neutropenia were 80% and 97% for dogs and 100% and 80% for cats; to detect anemia were 100% and 80% for dogs and 100% and 66% for cats; and to detect thrombocytopenia were 86% and 95% for dogs and 50% and 87% for cats.
Conclusions and Clinical Relevance—The point-of-care analyzer was reliable for monitoring CBCs of dogs and cats receiving chemotherapy. It had good to excellent correlation for WBC and neutrophil counts and Hct and accurately detected leukopenia, neutropenia, and anemia. Sensitivity of the analyzer for detecting thrombocytopenia was lower but acceptable.
OBJECTIVE To assess changes in biochemical and biophysical properties of canine RBCs during cold (1° to 6°C) storage in a licensed RBC additive solution (the RBC preservation solution designated AS-1) supplemented with ascorbic acid.
SAMPLE Blood samples from 7 neutered male Greyhounds; all dogs had negative results when tested for dog erythrocyte antigen 1.1.
PROCEDURES Blood was collected into citrate-phosphate-dextrose and stored in AS-1. Stored RBCs were supplemented with 7.1mM ascorbic acid or with saline (0.9% NaCl) solution (control samples). Several biochemical and biophysical properties of RBCs were measured, including percentage hemolysis, oxygen-hemoglobin equilibrium, and the kinetic rate constants for O2 dissociation, carbon monoxide association, and nitric oxide dioxygenation.
RESULTS Greyhound RBCs stored in AS-1 supplemented with ascorbic acid did not have significantly decreased hemolysis, compared with results for the control samples, during the storage period.
CONCLUSIONS AND CLINICAL RELEVANCE In this study, ascorbic acid did not reduce hemolysis during storage. Several changes in stored canine RBCs were identified as part of the hypothermic storage lesion.