Search Results

You are looking at 1 - 10 of 15 items for

  • Author or Editor: Colette Wagner-Mann x
  • Refine by Access: All Content x
Clear All Modify Search

Abstract

OBJECTIVE

To compare the acute strength (failure load and work to failure) of standard incisional gastropexy (SIG) and modified incisional gastropexy (MIG).

ANIMALS

37 pig cadavers.

PROCEDURES

Stomachs and right abdominal walls were harvested from pigs euthanized for reasons unrelated to this study. The tissues were stored in lactated Ringer’s solution overnight in a 5 °C cooler. Matching body wall and stomach tissue pairs were randomized and divided into 2 groups, on which either SIG or MIG was performed the following day. The MIG technique was identical to SIG except 2 additional simple interrupted sutures, 1 cranial and 1 caudal to the continuous suture line, were placed full thickness into the stomach to ensure engagement of the submucosa. After gastropexy, the samples underwent biomechanical testing. Information regarding change in position and load was generated by the MTESTQuattro software. Mode of failure was examined after the procedure was complete.

RESULTS

The MIG had higher failure load and work to failure compared to SIG. All failures were caused by gastric tissue tearing.

CLINICAL RELEVANCE

The MIG is biomechanically superior to SIG and may provide more security than SIG during healing. However, clinical study is needed to ascertain if there is a difference in gastropexy failure and complications between these 2 techniques.

Full access
in Journal of the American Veterinary Medical Association

Summary

Medical records of 3 cats and 72 dogs that had a fishhook endoscopically or surgically retrieved from the stomach or esophagus were reviewed. Endoscopic retrieval was successful in 41 of 62 (66%) animals, and retrieval time and hospitalization time for endoscopic retrieval were significantly shorter than times for surgical retrieval. Rate of failure of endoscopic retrieval was higher for animals with treble-barb, rather than single-barb, fishhooks. Whether a fishhook could be successfully retrieved endoscopically was independent of body weight, amount of time the fishhook had been present, location of the hook, and orientation within the esophagus.

Free access
in Journal of the American Veterinary Medical Association

Abstract

Objective—To determine whether lipid particle coalescence develops in veterinary parenteral nutrition (PN) admixture preparations that are kept at room temperature (23°C) for > 48 hours and whether that coalescence is prevented by admixture filtration, refrigeration, or agitation.

Sample Population—15 bags of veterinary PN solutions.

Procedures—Bags of a PN admixture preparation containing a lipid emulsion were suspended and maintained under different experimental conditions (3 bags/group) for 96 hours while admixtures were dispensed to simulate IV fluid administration (rate, 16 mL/h). Bags were kept static at 4°C (refrigeration); kept at 23°C (room temperature) and continuously agitated; kept at room temperature and agitated for 5 minutes every 4 hours; kept static at room temperature and filtered during delivery; or kept static at room temperature (control conditions). Admixture samples were collected at 0, 24, 48, 72, and 96 hours and examined via transmission electron microscopy to determine lipid particle diameters. At 96 hours, 2 samples were collected at a location distal to the filter from each bag in that group for bacterial culture.

Results—Distribution of lipid particle size in the control preparations and experimentally treated preparations did not differ significantly. A visible oil layer developed in continuously agitated preparations by 72 hours. Bacterial cultures of filtered samples yielded no growth.

Conclusions and Clinical Relevance—Data indicated that the veterinary PN admixtures kept static at 23°C are suitable for use for at least 48 hours. Manipulations of PN admixtures appear unnecessary to prolong lipid particle stability, and continuous agitation may hasten lipid breakdown.

Full access
in American Journal of Veterinary Research

Abstract

Objective—To compare measurements of body temperature obtained with auricular thermometers versus rectal thermometers in dogs with otitis externa.

Design—Prospective study.

Animals—100 client-owned dogs: 50 with and 50 without clinical evidence of otitis externa.

Procedure—Dogs were evaluated for the presence of otitis externa on the basis of clinical signs, otoscopic examination, and cytologic evaluation of ear exudate. Auricular and rectal temperatures were obtained simultaneously in all dogs prior to and following ear examination.

Results—There was a high correlation between auricular and rectal temperatures in dogs with otitis externa both prior to and after ear manipulation. Significant differences were not detected in temperature measurements among dogs with different degrees of otitis externa.

Conclusions and Clinical Relevance—Auricular temperature readings obtained by use of an auricular thermometer in dogs with otitis externa are accurate measurements of body temperature, compared with rectal temperature measurements. Temperature measurements are reliable before and after examination of the ear canal. (J Am Vet Med Assoc 2002; 221:378–380)

Full access
in Journal of the American Veterinary Medical Association

Abstract

Objective—To assess the reliability and accuracy of a predictive rectal thermometer, an infrared auricular thermometer designed for veterinary use, and a subcutaneous temperature-sensing microchip for measurement of core body temperature over various temperature conditions in dogs.

Design—Prospective study.

Animals—8 purpose-bred dogs.

Procedures—A minimum of 7 days prior to study commencement, a subcutaneous temperature-sensing microchip was implanted in 1 of 3 locations (interscapular, lateral aspect of shoulder, or sacral region) in each dog. For comparison with temperatures measured via rectal thermometer, infrared auricular thermometer, and microchip, core body temperature was measured via a thermistor-tipped pulmonary artery (TTPA) catheter. Hypothermia was induced during anesthesia at the time of TTPA catheter placement; on 3 occasions after placement of the catheter, hyperthermia was induced via administration of a low dose of endotoxin. Near-simultaneous duplicate temperature measurements were recorded from the TTPA catheter, the rectal thermometer, auricular thermometer, and subcutaneous microchips during hypothermia, euthermia, and hyperthermia. Reliability (variability) of temperature measurement for each device and agreement between each device measurement and core body temperature were assessed.

Results—Variability between duplicate near-simultaneous temperature measurements was greatest for the auricular thermometer and least for the TTPA catheter. Measurements obtained by use of the rectal thermometer were in closest agreement with core body temperature; for all other devices, temperature readings typically underestimated core body temperature.

Conclusions and Clinical Relevance—Among the 3 methods of temperature measurement, rectal thermometry provided the most accurate estimation of core body temperature in dogs.

Full access
in Journal of the American Veterinary Medical Association

Objective

To evaluate the in vitro axial extraction forces necessary to remove pins and to evaluate mechanical trauma resulting from pin insertion, using various types of pins and insertion techniques.

Design

Prospective, controlled study.

Subjects

Femurs of cadavers of dogs.

Procedure

Pins were inserted as follows: 1 non-threaded pin without drilling of a pilot hole, 1 enhanced threaded pin with drilling of a pilot hole, and 1 enhanced threaded pin without drilling of a pilot hole. After pin insertion, mechanical damage and proper pin insertion was determined by means of radiography. Axial extraction forces were determined for all pins, using a universal testing machine. Mechanical damage was evaluated in 12 additional femurs. After pin insertion, all pins were removed from the bone by use of a low-speed power drill. Samples were sectioned, processed, and evaluated by use of dissecting and scanning electron microscopy.

Results

Using radiography, a significant difference was detected in the number of periosteal trans-cortex fractures between the enhanced threaded and non-threaded pins. Axial extraction force was not significantly different between the enhanced threaded pins, regardless of insertion technique; however, the axial extraction force was significantly greater for enhanced threaded pins, compared with that for nonthreaded pins. Microfractures only were detected on the periosteum of the trans-cortex of enhanced threaded pins by use of scanning electron microscopy.

Clinical Implications

We cannot recommend a particular insertion technique to decrease mechanical trauma to the bone and to increase axial extraction force needed for removal of enhanced threaded pins from the femur of dogs. (J Am Vet Med Assoc 1996;208:883–887)

Free access
in Journal of the American Veterinary Medical Association

Summary

Eight dogs were determined to be orthopedically normal on the basis of prelavage physical examination, stifle radiography, synovial fluid analysis, and force plate analysis (peak vertical force normalized for body weight, and time on the force plate). Each dog had 1 stifle randomly assigned to be lavaged with 100 ml of a commercially available 0.05% (w/v) chlorhexidine diacetate solution, and the contralateral stifle was lavaged with lactated Ringer’s solution.

Difference was not detected between the chlorhexidine diacetate and lactated Ringer’s solution-treated joints, with regard to results of synovial fluid analysis and clinical lameness evaluations on days 4 and 8 after lavage. Chlorhexidine diacetate caused a more intense synovitis than did lactated Ringer’s solution, as determined by histologic evaluation of synovial membrane specimens after necropsy on day 8; however, a difference in the intensity of toluidine blue staining of articular cartilage was not found between treatments. Chlorhexidine diacetate, as a 0.05% (w/v) solution, cannot be recommended as a joint lavage fluid until the duration of inflammatory changes in the synovial membrane are determined or until the chemical constituents of chlorhexidine diacetate causing the synovitis can be identified and removed.

Free access
in American Journal of Veterinary Research

Abstract

Objective—To evaluate effects of protamine zinc insulin (PZI) on control of glycemia in cats with newly diagnosed diabetes mellitus or poorly controlled diabetes.

Design—Clinical trial.

Animals—67 diabetic cats.

Procedure—34 cats with newly diagnosed diabetes and 33 cats with poorly controlled diabetes were treated with PZI twice daily for 45 days. Control of glycemia was assessed on days 7, 14, 30, and 45 by evaluation of clinical response, change in body weight, serum fructosamine concentration, blood glucose concentration measured 1, 3, 5, 7, and 9 hours after administration of PZI, lowest blood glucose concentration, and mean blood glucose concentration during the 9-hour period after administration. Adjustments in dosage of PZI were made as needed to attain control of glycemia.

Results—For all cats, a significant increase in mean dosage of PZI and significant decreases in 9-hour mean blood glucose concentration, lowest mean blood glucose concentration, and mean serum fructosamine concentration were detected. For cats with poorly controlled diabetes, 9-hour mean blood glucose concentration and mean serum fructosamine concentration were significantly decreased on day 45, compared with day 0. Ninety percent of owners reported improvement or resolution of clinical signs by day 45.

Conclusions and Clinical Relevance—Results suggest that PZI was effective for control of glycemia in cats with newly diagnosed or poorly controlled diabetes and may be used as an initial treatment or as an alternative treatment in cats that do not respond to treatment with other types of insulin. ( J Am Vet Med Assoc 2001;218:38–42)

Full access
in Journal of the American Veterinary Medical Association

Abstract

Objective

To determine effects of glucosamine and acetylsalicylate on canine chondrocytes in 3-dimensional culture.

Sample Population

Chondrocytes isolated from articular cartilage of 2 adult female dogs recently euthanatized for reasons unrelated to orthopedic abnormalities.

Procedure

Chondrocytes were cultured in a 3-dimensional agarose-based medium alone (control), with glucosamine (100 μg/ml; GL), or with acetylsalicylate (18 µg/ml; AS). Supernatant and agarose plugs from 4 wells/group/d were collected on days 3, 6, and 12 of culture. Agarose plugs were evaluated for percentage of viable cells, percentage of cells producing pericellular or territorial matrix, glycosaminoglycan (GAG) concentration, and type-II collagen production. Prostaglandin E2 concentration in supernatants was determined.

Results

Chondrocytes in all groups had characteristics indicative of viability and differentiation; however, on day 12, a lower percentage of viable cells was detected in the AS group, compared with the other 2 groups. On day 6, GAG concentration in the AS group was significantly greater than concentrations in the other 2 groups. On day 12, GAG concentrations in the GL and AS groups were significantly less than in the control group. Within the GL and AS groups, cell viability was significantly less on day 12, compared with day 3. Significant differences in PGE2 concentration among or within groups and evidence of type II collagen production were not detected.

Conclusions

3-dimensional culture of canine chondrocytes allows for production of hyaline cartilage matrix constituents and growth of cells with morphologic characteristics similar to those of articular cartilage. Acetylsalicylate and glucosamine, at the single concentration evaluated, had detrimental effects on chondrocyte viability, GAG production, or both. (Am J Vet Res 1999;60:1546–1551)

Free access
in American Journal of Veterinary Research

Objective—

To determine the effect of treatment with recombinant human granulocyte colony-stimulating factor (rhG-CSF) for puppies with neutropenia secondary to canine parvovirus infection.

Design—

Randomized controlled clinical trial.

Animals—

23 puppies.

Procedure—

Diagnosis was confirmed by use of an ELISA for detection of canine parvovirus antigen in feces, and all puppies received standard treatment for parvoviral enteritis. All puppies had neutropenia (< 1,000 neutrophils/μl) at the time of admission to the hospital or within 4 days afterward. Eleven puppies were treated with rhG-CSF daily until neutrophil count was > 1,500 cells/μl; the remaining 12 puppies were not treated with rhG-CSF.

Results—

We did not detect any significant differences between groups regarding duration of hospitalization, neutrophil count when neutropenia was first detected, lowest neutrophil count, or time until neutrophil count was > 1,500 cells/μl.

Clinical Implications—

Results suggest that treatment with rhG-CSF may not be beneficial in puppies with neutropenia secondary to canine parvovirus infection. (J Am Vet Med Assoc 1998:213:991-992)

Free access
in Journal of the American Veterinary Medical Association