Objective—To determine whether Mycoplasma
strains typically associated with mastitis in dairy cattle
can be isolated from body sites other than the
Design—Prospective clinical trial.
Animals—7 Holstein cows in various stages of lactation
with intramammary Mycoplasma infection.
Procedure—Milk samples, antemortem swab specimens
from various body sites, and postmortem swab
and tissue specimens were submitted for Mycoplasma
culture. Pulsed-field gel electrophoresis (PFGE) was
performed on chromosomal digests of all Mycoplasma
isolates. Isolates with the same number and size of
chromosomal digest bands were considered to be of
the same type.
Results—For each cow, all isolates obtained from
milk, mammary gland parenchyma, and supramammary
lymph nodes had the same PFGE pattern. All
cows had at least 1 isolate from nonmammary system
tissues that had the same PFGE pattern as isolates
from the mammary system. Overall, 44 of the
70 (63%) Mycoplasma isolates obtained from body
sites other than mammary system sites had the
same PFGE pattern as did mammary system isolates.
Conclusions and Clinical Relevance—Results confirmed
our hypothesis that Mycoplasma strains isolated
from the milk of dairy cattle with Mycoplasma
mastitis frequently have PFGE patterns identical to
those for strains isolated from other body sites, suggesting
that there is at least a potential for internal
transmission of Mycoplasma organisms. (J Am Vet
Med Assoc 2005;227:455–459)
Objective—To compare plasma disposition of alkaloids
after lupine challenge in cattle that had given
birth to calves with lupine-induced arthrogryposis and
cattle that had given birth to clinically normal calves
and determine whether the difference in outcome
was associated with differences in plasma disposition
Animals—6 cows that had given birth to calves with
arthrogryposis and 6 cows that had given birth to clinically
normal calves after being similarly exposed to
lupine during pregnancy.
Procedure—Dried lupine (2 g/kg) was administered
via gavage. Blood samples were collected before and
at various time points for 48 hours after lupine administration.
Anagyrine, 5,6-dehydrolupanine, and lupanine
concentrations in plasma were measured by use
of gas chromatography. Plasma alkaloid concentration
versus time curves were generated for each alkaloid,
and pharmacokinetic parameters were determined
for each cow.
Results—No significant differences in area under the
plasma concentration versus time curve, maximum
plasma concentration, time to reach maximum plasma
concentration, and mean residence time for the 3
alkaloids were found between groups.
Conclusions and Clinical Relevance—Because no
differences were found in plasma disposition of
anagyrine following lupine challenge between cattle
that had given birth to calves with arthrogryposis and
those that had not, our findings do not support the
hypothesis that between-cow differences in plasma
disposition of anagyrine account for within-herd differences
in risk for lupine-induced arthrogryposis.
(Am J Vet Res 2004;65:1580–1583)