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In the fall of 2016, a 19-month-old 25-kg (55-lb) spayed female crossbred German Shepherd Dog was evaluated because of sudden onset of lethargy and inappetence. In March of the same year, the dog had undergone endoscopic biopsy, and examination of the specimen resulted in a diagnosis of eosinophilic inflammatory bowel disease (IBD). At the time of the evaluation, IBD was being managed medically. This dog had regular access to the outdoors and was known to consume coyote feces. Regular deworming was performed including administration of a 14-day course of fenbendazole (50 mg/kg [22.7 mg/lb], PO, q 24 h) in

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in Journal of the American Veterinary Medical Association

Abstract

Objective—To determine the impact of antimicrobial-containing semen extender on the growth of Taylorella equigenitalis in semen culture-positive for contagious equine metritis (CEM) and the development of CEM after artificial insemination with CEM-positive semen extended with antimicrobial-containing semen extender.

Design—Prospective study.

Animals—21 mature mares free of CEM, 1 mature stallion experimentally infected with CEM, and semen from a stallion naturally infected with CEM.

Procedures—CEM-positive semen was incubated in semen extender with and without antimicrobials (amikacin [final concentration, 1 g/L] and penicillin G potassium [0.63 g/L]) followed by determination of the number of colony-forming units of T equigenitalis. Mares were inseminated with raw, extended, or cryopreserved semen culture-positive for T equigenitalis and observed for clinical signs of CEM. Samples for bacterial culture were obtained from the uterus, clitoral sinuses, and clitoral fossa of mares 7, 14, and 21 days after artificial insemination.

Results—Antimicrobial-containing semen extender significantly reduced the number of colony-forming units of T equigenitalis in CEM-positive semen. Artificial insemination with raw CEM-positive semen resulted in clinical signs of CEM, whereas artificial insemination with extended or cryopreserved CEM-positive semen did not result in clinical signs of CEM.

Conclusions and Clinical Relevance—Antimicrobial-containing semen extender significantly reduced the risk of dissemination of CEM. The inclusion of amikacin (1 g/L) and penicillin G potassium (0.63 g/L) in extended semen reduced the transmission of CEM from stallions to mares during artificial insemination, which may result in altered dissemination of the disease.

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in Journal of the American Veterinary Medical Association