Objective—To detect and partially characterize papillomavirus (PV) DNA in squamous cell carcinoma (SCC) tumor specimens from cats.
Sample Population—54 formalin-fixed paraffinembedded skin biopsy specimens were examined. Specimens originated from Bowenoid in situ SCC (BISC; n = 21), invasive SCC (22), and skin affected by miscellaneous nonneoplastic conditions (11).
Procedures—Samples from each tissue block underwent DNA extraction after deparaffinization, and PCR assays were performed. Two sets of primers derived from PV E1 were used. The first set of primers was designed for the narrow-range PCR assay and was able to generate amplification products of feline PV (FePV), canine oral PV, or closely related PVs. The second set of primers was selected for the broad-range PCR assay because of its ability to amplify DNA from 64 human PVs. Sequence analysis of each amplified DNA was performed.
Results—1 of the 21 specimens of BISC was positive for PV DNA on the basis of narrow-range PCR assay results, whereas all the other specimens (BISC, invasive SCC, and controls) had negative results for PV DNA. In contrast, 5 of 21 BISC specimens and 4 of 22 invasive SCC specimens were positive for PV DNA on the basis of broad-range PCR assay results. Sequence analysis revealed that only 1 specimen was infected by a virus closely related to classic FePV. In the 8 other specimens positive for PV DNA, DNA of unknown PVs was uncovered.
Conclusions and Clinical Relevance—Bowenoid in situ SCC and invasive SCC of cats may be associated with PVs of genetic diversity.
Objective—To determine whether cyclosporine Ainduced
hyperplastic skin lesions of dogs were associated
with papillomavirus infections.
Animals—9 dogs that were treated with cyclosporine
A and developed hyperplastic skin lesions.
Procedure—History and clinical and histopathologic
data were collected. Paraffin-embedded skin biopsy
specimens from hyperplastic skin lesions were
immunostained for common papillomavirus genusspecific
structural antigens by use of a polyclonal rabbit
anti-bovine papillomavirus type 1 antiserum.
Sections from each tissue block underwent DNA
extraction, and polymerase chain reaction (PCR)
assays were performed with several sets of primers
to amplify a wide range of papillomavirus DNA from
humans and other animals.
Results—In 7 of 9 dogs, there were more than 10
hyperplastic skin lesions that microscopically resembled
those of psoriasiform lichenoid dermatosis. In
those dogs, results of testing for papillomavirus via
immunohistochemical analyses and PCR assays were
negative. In the other 2 dogs, there were only 1 and
3 verrucous lesions, and in those dogs, histologic
evaluation revealed koilocytes and nuclear viral inclusions
that were immunoreactive for papillomavirus
antigens. Papillomavirus DNA was amplified from
both dogs. One of the sequences was characteristic
for the canine oral papillomavirus, whereas the other
had similarities with the recently described canine
Conclusions and Clinical Relevance—In dogs,
hyperplastic skin lesions occasionally develop during
treatment with cyclosporine A. Most of the lesions
resemble those of psoriasiform lichenoid dermatosis,
although papillomavirus can be detected in some
instances. (Am J Vet Res 2005;66:1764–1769)
Objective—To evaluate the use of recombinant human (rh) thyroid-stimulating hormone (TSH) in dogs with suspected hypothyroidism.
Animals—64 dogs with clinical signs of hypothyroidism.
Procedures—Dogs received rhTSH (75 μg/dog, IV) at a dose independent of their body weight. Blood samples were taken before and 6 hours after rhTSH administration for determination of total serum thyroxine (T4) concentration. Dogs were placed into 1 of 3 groups as follows: those with normal (ie, poststimulation values indicative of euthyroidism), unchanged (ie, poststimulation values indicative of hypothyroidism; no thyroid gland stimulation), or intermediate (ie, poststimulation values between unchanged and normal values) post-TSH T4 concentrations. Serum canine TSH (cTSH) concentration was determined in prestimulation serum (ie, before TSH administration).
Results—14, 35, and 15 dogs had unchanged, normal, and intermediate post-TSH T4 concentrations, respectively. Basal T4 and post-TSH T4 concentrations were significantly different among groups. On the basis of basal serum T4 and cTSH concentrations alone, 1 euthyroid (normal post-TSH T4, low basal T4, and high cTSH concentrations) and 1 hypothyroid dog (unchanged post-TSH T4 concentration and low to with-in reference range T4 and cTSH concentrations) would have been misinterpreted as hypothyroid and euthyroid, respectively. Nine of the 15 dogs with intermediate post-TSHT4 concentrations had received medication known to affect thyroid function prior to the test, and 2 of them had severe nonthyroidal disease.
Conclusions and Clinical Relevance—The TSH-stimulation test with rhTSH is a valuable diagnostic tool to assess thyroid function in selected dogs in which a diagnosis of hypothyroidism cannot be based on basal T4 and cTSH concentrations alone.