Objective—To compare effects of sterilization with hydrogen peroxide gas plasma (HPGP), ethylene oxide, and steam on bioadhesive properties of nylon and polyethylene lines used for stabilization of canine stifle joints.
Sample—Samples of a 36.3-kg test nylon leader line, 57.8-kg test nylon fishing line, and 2-mm ultrahigh–molecular weight polyethylene (UHMWPE) were used.
Procedures—In this in vitro study, samples of nylon leader line, fishing line, and UHMWPE sterilized by use of HPGP, ethylene oxide, and steam or unsterilized samples were used. Bacterial adherence on unsterilized and sterilized samples was tested with Staphylococcus epidermidis and Escherichia coli. Five samples were examined for each line type and sterilization condition, and final colony counts were obtained.
Results—Bacterial adherence was significantly affected by method of sterilization for all 3 line types. For most of the samples, bacterial adherence was similar or lower when HPGP sterilization was used, compared with results for sterilization via ethylene oxide and steam, respectively. Bacterial adherence was significantly higher for UHMWPE, compared with adherence for the nylon line, regardless of the sterilization method used. Bacterial adherence was higher for nylon fishing line than for nylon leader line for S epidermidis after ethylene oxide sterilization and for E coli after HPGP and ethylene oxide sterilization.
Conclusions and Clinical Relevance—Effects of HPGP sterilization on bioadhesive properties of nylon and polyethylene lines compared favorably with those for ethylene oxide and steam sterilization. Also, nylon line may be a more suitable material than UHMWPE for suture prostheses on the basis of bacterial adherence properties.
Objective—To evaluate the effects of nephrotomy on
renal function in clinically normal cats.
Animals—20 specific-pathogen-free, 9- to 11-month old
female mixed-breed cats.
Procedure—Serum chemistry analyses, CBC determinations,
urinalyses, microbiologic urine cultures,
renal ultrasonography, abdominal radiography, and
single-kidney and total glomerular filtration rate (GFR)
determinations by use of renal scintigraphy and measurements
of plasma disappearance of technetium
99m-diethylenetriaminepentaacetic acid were performed
before surgery and at 3, 12, 26, 52, and 78
weeks after surgery in 10 cats that underwent unilateral
nephrotomy and in 10 control cats that underwent
a sham surgical procedure.
Results—Two cats (1 from each group) did not complete
the study, and their data were eliminated from
analyses. Unilateral nephrotomy resulted in a 10% to
20% reduction in mean single-kidney GFR, compared
with that of nephrotomy contralateral control kidneys.
However, mean total GFR in nephrotomy-group cats
was not significantly different from that of shamgroup
cats. Over the 78 weeks of study, mean total
GFR declined 34% and 40% in nephrotomy- and
sham-group cats, respectively. Adverse events associated
with nephrotomy included persistent microscopic
hematuria, renal pelvis hyperechogenicity with
distant shadowing on ultrasonographic evaluation,
dilatation of renal pelves, and hydronephrosis.
Conclusions and Clinical Relevance—Nephrotomy
in normal functioning feline kidneys results in a modest
relative reduction in renal function, compared with
contralateral kidney controls, but has minimal effect
on total GFR when compared with sham-operated
control cats. However, any detrimental effects of
nephrotomy may be magnified in cats with diseased
kidneys, which may have little or no capacity for repair
or compensation. (Am J Vet Res 2005;66:1400–1407)