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  • Author or Editor: Candace Bourne x
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Abstract

Objective—To determine the nucleotide sequence of the α IIb gene from canine platelet-derived cDNA.

Animals—3 adult dogs.

Procedure—First-strand cDNA was prepared from total RNA isolated from canine platelets. The cDNA was amplified, using specific primers in polymerase chain reaction (PCR), and the nucleotide sequence was obtained from purified PCR products.

Results—Except for the nucleotide at position 694, results of all sequencing reactions of α IIb were identical for canine platelet-derived cDNA. Canine α IIb had 3 fewer codons than α IIb of humans. The nucleotide and deduced amino acid sequences of full-length canine α IIb shared ≥ 83% similarity with the sequences established for humans. Segments of canine α IIb nucleotide and deduced amino acid sequences were ≥ 78% similar to α IIb associated with 7 functional domains (extracellular, transmembrane, cytoplasmic, and 4 calcium-binding domains) in humans, with the highest degree of similarity correlating with the sequences of the 4 calcium-binding domains. Amino acid residues associated with development of alloantibodies in humans (Met837, Val837, Ile843, Ser843) are not encoded by canine α IIb .

Conclusions and Clinical Relevance—The nucleotide variation at position 694 of canine α IIb may represent a polymorphism. The species differences in the α IIb sequence may contribute to variations in receptor-li gand interactions. The high degree of α IIb sequence conservation of the 4 calcium-binding domains implies functional importance. Some disorders associated with α IIb β3 in dogs are clinically analogous to diseases in humans, and results indicate that dogs are an appropriate model for the evaluation of gene therapy and other treatments of platelet-associated disorders. (Am J Vet Res 2001;62:1486–1492)

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in American Journal of Veterinary Research

Abstract

Objective—To identify subclinical Babesia gibsoni infection in American Pit Bull Terriers from the southeastern United States and to determine the genetic sequence of parasite DNA isolated from these dogs.

Design—Case series.

Animals—33 American Pit Bull Terriers and 87 dogs of various other breeds.

Procedure—Blood smears were examined for microscopic evidence of the parasite, and DNA was extracted from blood samples and used in a polymerase chain reaction (PCR) assay designed to amplify the small subunit ribosomal RNA gene sequence of B gibsoni. Amplification products of the expected size were sequenced, and sequences were compared with published sequences for B gibsoni isolates. Hematocrit, platelet count, mean platelet volume, WBC count, and eosinophil count were compared between dogs with positive PCR assay results and dogs with negative results.

Results—Results of the PCR assay were positive for 18 of the 33 (55%) American Pit Bull Terriers, including all 10 dogs with microscopic evidence of parasitemia. Only 1 of these dogs was clinically ill at the time blood samples were collected. Results of microscopic evaluation of blood smears and of the PCR assay were negative for the 87 other dogs. Hematocrit and platelet count were significantly lower in dogs with positive PCR assay results than in dogs with negative results.

Conclusions and Clinical Relevance—Results suggest that American Pit Bull Terriers in the southeastern United States may be subclinically infected with B gibsoni. However, subclinical infection was not identified in dogs of other breeds from the same geographic area. (J Am Vet Med Assoc 2002;220: 325–329)

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in Journal of the American Veterinary Medical Association

Abstract

Objective—To determine whether a polymerase chain reaction (PCR) assay could be used to detect Eperythrozoon wenyoniin the blood of cattle.

Design—Prospective study.

Animals—95 cattle from various herds in Alabama and Georgia and 96 bulls enrolled in Auburn University's Alabama Beef Cattle Improvement Association Bull Test program.

Procedure—Blood samples were collected by means of venipuncture of the median caudal vein and submitted for a CBC and PCR assay. Blood smears were made immediately after blood collection and examined by means of light microscopy.

Results—Three of 95 cattle from herds in Alabama and Georgia and 5 of 96 bulls enrolled in the Bull Test program had positive PCR assay results. Organisms were seen in blood smears from only 5 of these 8 animals. Organisms were not seen in blood smears from any animals for which results of the PCR assay were negative.

Conclusions and Clinical Relevance—Results suggest that a PCR assay may be an effective method for detecting E wenyoni infection in cattle and that the PCR assay may be a more sensitive test than evaluation of blood smears. (J Am Vet Med Assoc 2001; 219:1432–1434)

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in Journal of the American Veterinary Medical Association