Objective—To evaluate effects of Carolina rinse solution,
dimethyl sulfoxide (DMSO), and 21-aminosteroid,
U-74389G, on microvascular permeability and
morphology of the equine jejunum after low-flow
ischemia and reperfusion.
Animals—20 healthy adult horses.
Procedure—Under anesthesia, full-thickness biopsy
specimens of a distal portion of the jejunum were
obtained for baseline measurements. In addition to a
control segment, 2 jejunal segments were identified
as sham-operated or experimental segments.
Experimental segments underwent 60 minutes of
low-flow ischemia and 3.5 hours of reperfusion.
Treatments were as follows: U-74389G (3 mg/kg, IV; 6
horses), DMSO (20 mg/kg, IV; 6) diluted in 1 L of saline
(0.9% NaCl) solution, local perfusion (via jejunal artery)
of Carolina rinse solution (0.5 mL/kg; 4), and local perfusion
of lactated Ringer's solution (0.5 mL/kg; 4).
Results—Jejunal microvascular permeability was
significantly lower after treatment with Carolina rinse
solution or DMSO, compared with U-74389G or lactated
Ringer's solution treatments. After DMSO
treatment, serosal- and submucosal-layer edema
was significantly increased in experimental segments,
compared with control or sham-operated
segments; however, edema increases were significantly
less than for lactated Ringer's solution or
U-74389G treatments. Significant decreases in
intestinal wet weight-to-dry weight ratio were found
following Carolina rinse solution or DMSO treatments,
compared with lactated Ringer's solution or
U-74389G treatments. Edema formation and leukocyte
infiltration in jejunal segments of horses treated
with lactated Ringer's solution or U-74389G were
increased, compared with Carolina rinse solution or
Conclusions and Clinical Relevance—Carolina rinse
solution and DMSO may be protective against
ischemia-reperfusion injury in the equine jejunum.
(Am J Vet Res 2005;66:525–536)