Objective—To investigate the possibility that variants
in the acidic or basic keratin genes or in desmoglein 1
may cause the clinical manifestation of familial footpad
hyperkeratosis in Irish Terriers.
Animals—11 dogs belonging to 2 related affected
pedigrees of Irish Terriers.
Procedure—Genomic DNA was extracted from blood
samples obtained from each dog. The DNA markers
linked to the genes keratin 2, keratin 9, and
desmoglein 1 were amplified by use of a polymerase
chain reaction technique, and length of the products
was determined by use of an automatic DNA analyzer.
Results—All tested markers yielded information.
None of the markers (genotype) cosegregated with
the clinical status of the dogs (phenotype) in the 2
Conclusions and Clinical Relevance—Mutations in
the genes encoding keratin 2 and 9 as well as
desmoglein 1 are highly unlikely to be the primary
cause of familial footpad hyperkeratosis in Irish
Terriers. (Am J Vet Res 2003;64:715–720)
Objective—To evaluate the role of the phospholamban
gene in purebred large-breed dogs with dilated
Animals—6 dogs with DCM, including 2 Doberman
Pinschers, 2 Newfoundlands, and 2 Great Danes.
Procedure—All dogs had clinical signs of congestive
heart failure, and a diagnosis of DCM was made on
the basis of echocardiographic findings. Blood samples
were collected from each dog, and genomic
DNA was isolated by a salt extraction method.
Specific oligonucleotides were designed to amplify
the promoter, exon 1, the 5'-part of exon 2 including
the complete coding region, and part of intron 1 of the
canine phospholamban gene via polymerase chain
reaction procedures. These regions were screened
for mutations in DNA obtained from the 6 dogs with
Results—No mutations were identified in the promoter,
5' untranslated region, part of intron 1, part of
the 3' untranslated region, and the complete coding
region of the phospholamban gene in dogs with
Conclusions and Clinical Relevance—Results indicate
that mutations in the phospholamban gene are
not a frequent cause of DCM in Doberman Pinschers,
Newfoundlands, and Great Danes. (Am J Vet Res 2005;66:432–436)