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- Author or Editor: Barrett D. Slenning x
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Objective
To determine whether various peripar-turient events or 305-day milk production during the previous lactation period were associated with abomasal displacement in dairy cows.
Design
Retrospective, case-control study.
Animals
75 pairs of case and control cows from 3 university-owned and 3 commercial dairy herds.
Procedure
Cows with abomasal displacement were matched with control cows on the basis of herd of origin, breed, age, and calving date. Frequency of specific periparturient events during the period from 2 weeks prior to parturition to diagnosis of abomasal displacement, as well as milk production during the preceding lactation period, were compared between case and control cows.
Results
Multivariate analyses indicated that case cows were significantly more likely to have had retained placenta, ketosis, a stillborn calf, metritis, twins, or parturient paresis than were control cows. Dystocia, mastitis, and milk production during the previous lactation period were not associated with abomasal displacement.
Clinical Implications
Results indicated that a variety of periparturient events were associated with development of abomasal displacement among dairy cows. (J Am Vet Med Assoc 1999;214:1660-1663)
Abstract
Objective
To determine effects of fecal sample storage and delayed secondary enrichment (DSE) on detection of Salmonella spp in swine feces.
Sample Population
Fecal samples obtained from 84 pigs in a commercial herd.
Procedure
Each fecal sample underwent 3 storage treatments: no storage (ie, processed on the day of collection), storage at 4 C for 6 days, and storage at −15 C for 14 days. After assigned storage treatments, all samples were enriched in Rappaport-Vassiladias (RV) broth (single enrichment) and plated on XLT4 agar. Delayed secondary enrichment was performed, using single enrichment broths that were stored for 4 days at room temperature.
Results
Of 504 cultures, 186 (36.9%) were Salmonella positive. A difference in proportions of samples with positive results was not found between same-day processing and storage at 4 C for 6 days. Compared with use of single enrichment for 24 hours (34% positive), use of DSE resulted in a greater proportion (40%; P < 0.001) of samples with positive results. Estimated relative sensitivities for the storage methods were 0.90, 0.85, and 0.71 for same-day processing, storage at 4 C for 6 days, and storage at −15 C for 14 days, respectively.
Conclusions
Where practical, processing of fecal samples on the day of collection is recommended, although storage at 4 C for several days does not result in marked loss of sensitivity. Improved detection associated with DSE warrants further investigation and optimization. (Am J Vet Res 1999;60:359–362)
