Objective—To determine the characteristics of an automated canine C-reactive protein (CRP) assay and evaluate 2 human CRP assays for use in dogs.
Animals—56 client-owned dogs with pyometra and 11 healthy control dogs.
Procedures—Samples from 11 dogs with high (> 100 mg/L) or low (< 10 mg/L) CRP concentrations (determined by use of a canine ELISA) were evaluated by use of the automated canine CRP assay. Intra- and interassay imprecision was determined (by use of those 2 plasma pools), and assay inaccuracy was assessed by use of logistic regression analysis of results obtained via ELISA and the automated canine CRP assay. Two automated human CRP assays were used to measure plasma CRP concentration in 10 dogs.
Results—By use of the ELISA, mean ± SD plasma CRP concentration was 96.1 ± 38.5 mg/L and 10.1 ± 23.2 mg/L in dogs with pyometra and control dogs, respectively. The automated canine assay had intra-assay coefficients of variation (CVs) of 7.8% and 7.9%, respectively, and interassay CVs of 11.1% and 13.1%, respectively. Results from the automated assay were highly correlated with results obtained via ELISA. The human assay results did not exceed 0.4 mg/L in any dog.
Conclusions and Clinical Relevance—The automated canine CRP assay had less interassay imprecision, compared with the ELISA. The 2 human CRP assays were not suitable for analysis of canine plasma samples. The automated canine CRP assay was more precise than the ELISA for serial evaluations of plasma CRP concentration in dogs.
Objective—To characterize the bacteria of the genital
tract in adult cats; assess the effect of estrus, mating,
and administration of progestins on those microorganisms
in females; and evaluate whether results of
bacteriologic culture of vaginal swabs are affected by
cleansing of the vulva prior to sampling or by repeated
Animals—66 female and 29 male cats undergoing
routine ovariohysterectomy or castration.
Procedure—Specimens were obtained from vaginal
and uterine or preputial mucosae with swabs moistened
with sterile saline (0.9% NaCl) solution. In 9 cats, vaginal
specimens were obtained before and after cleansing of
the vulva with ethanol; in 7 female cats, 2 vaginal specimens
were obtained in immediate succession.
Results—Aerobic bacteria were most commonly isolated
from cats' vaginas and prepuces; anaerobic bacteria
were isolated frequently from males (41%) but
rarely from females (5%). Generally, culture results
were not affected by cleansing of the vulva or repeated
vaginal sampling. The bacterial population of the
vaginas of cats was influenced by stage of the
estrous cycle but not by mating or administration of
progestins. Bacteria were not isolated from the
uterus of any cat.
Conclusions and Clinical Relevance—In cats, bacteria
of the genital tract in females are predominantly
aerobic; in males, aerobic and anaerobic bacteria are
found. The bacterial population of the vagina is affected
by stage of the estrous cycle. Pure growth of bacteria
in culture of genital tract specimens is a normal
finding; antimicrobials should only be administered if
clinical signs of genital infection are present.
(Am J Vet Res 2003;64:963–968)