Objective—To identify a technique for measurement
of glycated hemoglobin percentage in blood samples
obtained from various species of nonhuman primates
(NHPs), to determine whether these percentages varied
with respect to glycemic control, and to assess
whether this physiologic variable provided a suitable
test for diagnosing diabetes mellitus in NHPs.
Sample Population—166 blood samples collected
from 121 NHPs comprising 22 species from the
Haplorhine and Strepsirhine suborders and including
nondiabetic, treated-diabetic, and diabetic animals in
23 zoologic institutions throughout the United States.
Procedure—Hemoglobin A1c percentage was measured
in 154 samples by use of high-performance liquid
chromatography. Total glycated hemoglobin percentage
was measured in 159 samples by use of a
boronate-affinity chromatographic assay. Glucose
concentration was measured in 157 samples with an
autochemical analyzer by use of a hexose kinase
Results—The boronate-affinity chromatographic technique
for measurement of total glycated hemoglobin
percentage was the most suitable method.
Nondiabetic Haplorhines had percentages higher than
those in nondiabetic Strepsirhines. In Haplorhines,
diabetic animals had percentages higher than those in
treated-diabetic animals, which had percentages higher
than those in nondiabetic animals. In Strepsirhines,
this pattern was less pronounced.
Conclusions and Clinical Relevance—Measurement
of total glycated hemoglobin percentage provides
useful information for diagnosing diabetes mellitus
in Haplorhines and, possibly, in Strepsirhines.
Until reference ranges are established for each
species, it is recommended that results for samples
from NHPs without clinical signs of diabetes mellitus
be compared with results of samples collected concomitantly
from NHPs with clinical signs of this condition.
( Am J Vet Res 2003;64:562–568)